Overview

  • Product nameAnti-HPV18 E2 antibody [2E7]
  • Description
    Mouse monoclonal [2E7] to HPV18 E2
  • SpecificityReacts in WB, and ELISA with HPV18 native E2 protein as well recombinant E2 protein expressed in bacteria and Cos 7 cells.
  • Tested applicationsSuitable for: WB, ELISAmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human Papillomavirus (HPV)
  • Immunogen

    Recombinant full length protein corresponding to HPV18 E2.

  • EpitopeMapped to amino acids 1-83.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesPurified from bacteria by affinity chromatography.
  • ClonalityMonoclonal
  • Clone number2E7
  • IsotypeIgG

Applications

Our Abpromise guarantee covers the use of ab100969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 µg/ml. Predicted molecular weight: 41 kDa.
ELISA Use a concentration of 0.1 µg/ml.
  • Application notesIs unsuitable for IP.
  • Target

    • RelevanceHuman papillomaviruses (HPV) are small DNA viruses which infect epithelia of the skin and mucosa. Over 90 types have been identified and they mostly cause a variety of benign lesions such as warts and verrucae. However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmed as agents which cause cervical cancer. Human Papillomavirus (HPV) E2 proteins are the major viral regulators of transcription and replication during the viral life cycle.
    • Cellular localizationHost nucleus
    • Database links
      • Alternative names
        • E2 antibody
        • Human papillomavirus type 18 E2 protein antibody
        • Regulatory protein E2 antibody

      Anti-HPV18 E2 antibody [2E7] images

      • All lanes : Anti-HPV18 E2 antibody [2E7] (ab100969) at 1/100 dilution

        Lane 1 : lysates of U20S cells transfected with plasmid expressing HPV11 E2 protein
        Lane 2 : lysates of plain U20S cells
        Lane 3 : lysates of plain HeLa cells
        Lane 4 : lysates of U20S transfected with plasmid expressing HPV11 E2 protein
        Lane 5 : marker


        Predicted band size : 41 kDa
      • Immunofluorescence (IF) analysis of HPV18 E2 protein in U2OS cells. All the nuclei were stained by DAPI. Green color represents nucleus localized HPV18 E2 detected by ab100969.

      References for Anti-HPV18 E2 antibody [2E7] (ab100969)

      ab100969 has not yet been referenced specifically in any publications.

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