Overview

  • Product name
  • Description
    Rabbit polyclonal to HRAS + KRAS
  • Specificity
    Detects endogenous levels of total KRAS/HRAS protein
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human HRAS + KRAS.

  • Positive control
    • HeLa cell extract

Properties

Applications

Our Abpromise guarantee covers the use of ab53160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 22 kDa).
ELISA 1/20000.

Target

  • Relevance
    KRAS: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation (PubMed:23698361, PubMed:22711838). Enzyme regulation: Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP). Interaction with SOS1 promotes exchange of bound GDP by GTP. HRAS: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Enzyme regulation: Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).
  • Database links
  • Alternative names
    • c-H-ras antibody
    • c-K-ras antibody
    • c-Ki-ras antibody
    • GTPase HRas antibody
    • GTPase KRas antibody
    • H-Ras-1 antibody
    • Ha-Ras antibody
    • HRAS antibody
    • HRAS1 antibody
    • K-Ras 2 antibody
    • Ki-Ras antibody
    • KRAS antibody
    • KRAS2 antibody
    • p21ras antibody
    • RASK2 antibody
    • Transforming protein p21 antibody
    see all

Images

  • All lanes : Anti-HRAS + KRAS antibody (ab53160) at 1/500 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : HeLa cell extract with the immunising peptide

    Lysates/proteins at 5 µg per lane.

    Secondary
    Lane 1 : Alkaline Phosphatase Goat Anti-Rabbit IgG (H+L)
    Lane 2 : Alkaline Phosphatase Goat Anti-Rabbit IgG (H+L)


    Predicted band size : 22 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)

References

ab53160 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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