Anti-HRG beta 1 antibody (ab87547)

Overview

  • Product nameAnti-HRG beta 1 antibody
    See all HRG beta 1 primary antibodies
  • Description
    Rabbit polyclonal to HRG beta 1
  • Tested applicationsSuitable for: IHC-P, Sandwich ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant Human HRG beta 1.

  • Positive control
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human normal liver.

Properties

  • FormLyophilised:Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab87547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to HRG beta 1 (Biotin) (ab87549). Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human HRG beta 1. Can be used as capture antibody when paired with ab87549.
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 70 kDa.

Target

  • RelevanceHRG beta 1 is an isoform from the Neuregulin/Heregulin family of structurally related polypeptide growth factors which are derived from alternatively spliced genes (NRG1, NRG2, NRG3 and NRG4). To date, there are over 14 soluble and transmembrane proteins derived from the NRG1 gene. The receptors for all NRG1 isoforms are the ERBB family of tyrosine kinase transmembrane receptors. Through interaction with ERBB receptors, NRG1 isoforms induce the growth and differentialtion of epithelial, neuronal, glial, and other types of cells. HRG1-ß1 contains an Ig domain and an EGF-like domain that is necessary for direct binding to receptor tyrosine kinases erb3 and erb4. Although HRG1-ß1 biological effects are still unclear, it has been found to promote motility and invasiveness of breast cancer cells which may also involve up-regulation of expression and function of the autocrine motility promoting factor (AMF).
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • Acetylcholine receptor inducing activity antibody
    • ARIA antibody
    • Breast cancer cell differentiation factor p45 antibody
    • GGF antibody
    • GGF2 antibody
    • glial growth factor antibody
    • Heregulin antibody
    • Heregulin beta1 antibody
    • heregulin, alpha (45kD, ERBB2 p185 activator) antibody
    • HGL antibody
    • HRG antibody
    • HRG beta 1A antibody
    • HRG1 antibody
    • HRGA antibody
    • NDF antibody
    • neu differentiation factor antibody
    • Neuregulin 1 antibody
    • NRG1 antibody
    • sensory and motor neuron derived factor antibody
    • SMDF antibody
    see all

Anti-HRG beta 1 antibody images

  • IHC image of HRG beta 1 staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab87547, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-HRG beta 1 antibody (ab87547)

ab87547 has not yet been referenced specifically in any publications.

Product Wall

I compared the homology of the sequence and Pro-neuregulin-1, membrane-bound isoforms (Q02297 NRG1_HUMAN). The only isoforms which had 100% were 6 (beta1, 71kDa) and 11 (Type IV-beta1a, 65,5kDa). 100%-isoforms 6,11 84%-isoforms 7 83%-isoforms 8...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"