Anti-HRG beta 1 antibody (Biotin) (ab87549)

Overview

  • Product name
    Anti-HRG beta 1 antibody (Biotin)
    See all HRG beta 1 primary antibodies
  • Description
    Rabbit polyclonal to HRG beta 1 (Biotin)
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: ELISA, Sandwich ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant Human HRG beta 1.

Properties

  • Form
    Lyophilised:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab87549 was purified by affinity chromatography and then biotinylated.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab87549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.25 - 1 µg/ml. (using 100 µl/well antibody solution). ab87549 in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant human HRG beta 1.
Sandwich ELISA Use a concentration of 0.25 - 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to HRG beta 1 (ab87547). When used as detection antibody when paired with recommended antibody, allows detection at least 0.2 - 0.4 ng/well of recombinant human HRG beta 1(using 100 µl/well antibody solution).
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 70 kDa. Used in conjunction with compatible secondary reagents the detection limit for recombinant human HRG beta 1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Target

  • Relevance
    HRG beta 1 is an isoform from the Neuregulin/Heregulin family of structurally related polypeptide growth factors which are derived from alternatively spliced genes (NRG1, NRG2, NRG3 and NRG4). To date, there are over 14 soluble and transmembrane proteins derived from the NRG1 gene. The receptors for all NRG1 isoforms are the ERBB family of tyrosine kinase transmembrane receptors. Through interaction with ERBB receptors, NRG1 isoforms induce the growth and differentialtion of epithelial, neuronal, glial, and other types of cells. HRG1-ß1 contains an Ig domain and an EGF-like domain that is necessary for direct binding to receptor tyrosine kinases erb3 and erb4. Although HRG1-ß1 biological effects are still unclear, it has been found to promote motility and invasiveness of breast cancer cells which may also involve up-regulation of expression and function of the autocrine motility promoting factor (AMF).
  • Cellular localization
    Secreted
  • Database links
  • Alternative names
    • Acetylcholine receptor inducing activity antibody
    • ARIA antibody
    • Breast cancer cell differentiation factor p45 antibody
    • GGF antibody
    • GGF2 antibody
    • glial growth factor antibody
    • Heregulin antibody
    • Heregulin beta1 antibody
    • heregulin, alpha (45kD, ERBB2 p185 activator) antibody
    • HGL antibody
    • HRG antibody
    • HRG beta 1A antibody
    • HRG1 antibody
    • HRGA antibody
    • NDF antibody
    • neu differentiation factor antibody
    • Neuregulin 1 antibody
    • NRG1 antibody
    • sensory and motor neuron derived factor antibody
    • SMDF antibody
    see all

References

ab87549 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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