Overview

  • Product name
  • Description
    Rabbit polyclonal to HRH3
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rhesus monkey
  • Immunogen

    Synthetic peptide: designeds within amino acids 257-287 of Human HRH3

  • Positive control
    • Jurkat

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab84468 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 49 kDa.
IP Use at an assay dependent concentration. PubMed: 24566390

Target

  • Function
    The H3 subclass of histamine receptors could mediate the histamine signals in CNS and peripheral nervous system. Signals through the inhibition of adenylate cyclase and displays high constitutive activity (spontaneous activity in the absence of agonist). Agonist stimulation of isoform 3 niether modified adenylate cyclase activity nor induced intracellular calcium mobilization.
  • Tissue specificity
    Expressed predominantly in the CNS, with the greatest expression in the thalamus and caudate nucleus. The various isoforms are mainly coexpressed in brain, but their relative expression level varies in a region-specific manner. Isoform 3 and isoform 7 are highly expressed in the thalamus, caudate nucleus and cerebellum while isoform 5 and isoform 6 show a poor expression. Isoform 5 and isoform 6 show a high expression in the amygdala, substantia nigra, cerebral cortex and hypothalamus. Isoform 7 is not found in hypothalamus or substantia nigra.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • G protein coupled receptor 97 antibody
    • G-protein coupled receptor 97 antibody
    • GPCR 97 antibody
    • GPCR97 antibody
    • H3R antibody
    • HH 3R antibody
    • HH3 R antibody
    • HH3R antibody
    • Histamine H3 receptor antibody
    • Histamine receptor H3 antibody
    • HRH 3 antibody
    • HRH3 antibody
    • HRH3_HUMAN antibody
    • OTTHUMP00000031459 antibody
    see all

Images

  • Anti-HRH3 antibody (ab84468) at 1/500 dilution + 35µg of Jurkat whole cell lysate

    Predicted band size : 49 kDa
    Observed band size : 49 kDa
    Additional bands at : 32 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Yan H  et al. Histamine H3 receptors aggravate cerebral ischaemic injury by histamine-independent mechanisms. Nat Commun 5:3334 (2014). WB, IP ; Mouse . Read more (PubMed: 24566390) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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