Recombinant
RabMAb

Anti-HRH3 antibody [EPR5631] (ab124732)

Overview

  • Product name
    Anti-HRH3 antibody [EPR5631]
    See all HRH3 primary antibodies
  • Description
    Rabbit monoclonal [EPR5631] to HRH3
  • Tested applications
    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human HRH3 (N terminal). The exact sequence is proprietary. (In the extracellular domain).
    Database link: Q9Y5N1

  • Positive control
    • Lysates of: Jurkat, SH-SY5Y, C6, A549, Neuro-2a Jurkat cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)
    KD = 2.62 x 10 -10 M
    Learn more about KD
  • Storage buffer
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR5631
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 49 kDa.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      The H3 subclass of histamine receptors could mediate the histamine signals in CNS and peripheral nervous system. Signals through the inhibition of adenylate cyclase and displays high constitutive activity (spontaneous activity in the absence of agonist). Agonist stimulation of isoform 3 niether modified adenylate cyclase activity nor induced intracellular calcium mobilization.
    • Tissue specificity
      Expressed predominantly in the CNS, with the greatest expression in the thalamus and caudate nucleus. The various isoforms are mainly coexpressed in brain, but their relative expression level varies in a region-specific manner. Isoform 3 and isoform 7 are highly expressed in the thalamus, caudate nucleus and cerebellum while isoform 5 and isoform 6 show a poor expression. Isoform 5 and isoform 6 show a high expression in the amygdala, substantia nigra, cerebral cortex and hypothalamus. Isoform 7 is not found in hypothalamus or substantia nigra.
    • Sequence similarities
      Belongs to the G-protein coupled receptor 1 family.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • G protein coupled receptor 97 antibody
      • G-protein coupled receptor 97 antibody
      • GPCR 97 antibody
      • GPCR97 antibody
      • H3R antibody
      • HH 3R antibody
      • HH3 R antibody
      • HH3R antibody
      • Histamine H3 receptor antibody
      • Histamine receptor H3 antibody
      • HRH 3 antibody
      • HRH3 antibody
      • HRH3_HUMAN antibody
      • OTTHUMP00000031459 antibody
      see all

    Images

    • All lanes : Anti-HRH3 antibody [EPR5631] (ab124732)

      Lane 1 : Jurkat cell lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : C6 cell lysate
      Lane 4 : A549 cell lysate
      Lane 5 : Neuro-2a cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 49 kDa
    • Flow cytometric analysis of Jurkat cells using ab124732 (red)at 1/100 dilution or a rabbit IgG (negative) (green)
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab124732 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Vielen Dank für Ihre Email. Ich werde Ihnen gerne einen unserer anderen HRH3 Antikörper als Austauschprodukt zukommen lassen.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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