Anti-HRH3 antibody [EPR5631] (ab124732)


  • Product nameAnti-HRH3 antibody [EPR5631]
    See all HRH3 primary antibodies
  • Description
    Rabbit monoclonal [EPR5631] to HRH3
  • Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human HRH3 (N terminal). The exact sequence is proprietary. (In the extracellular domain).
    Database link: Q9Y5N1

  • Positive control
    • Lysates of: Jurkat, SH-SY5Y, C6, A549, Neuro-2a Jurkat cells
  • General notes

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD) KD = 2.62 x 10 -10 M Learn more about KD
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEPR5631
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab124732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 49 kDa.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for IHC-P or IP.
  • Target

    • FunctionThe H3 subclass of histamine receptors could mediate the histamine signals in CNS and peripheral nervous system. Signals through the inhibition of adenylate cyclase and displays high constitutive activity (spontaneous activity in the absence of agonist). Agonist stimulation of isoform 3 niether modified adenylate cyclase activity nor induced intracellular calcium mobilization.
    • Tissue specificityExpressed predominantly in the CNS, with the greatest expression in the thalamus and caudate nucleus. The various isoforms are mainly coexpressed in brain, but their relative expression level varies in a region-specific manner. Isoform 3 and isoform 7 are highly expressed in the thalamus, caudate nucleus and cerebellum while isoform 5 and isoform 6 show a poor expression. Isoform 5 and isoform 6 show a high expression in the amygdala, substantia nigra, cerebral cortex and hypothalamus. Isoform 7 is not found in hypothalamus or substantia nigra.
    • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
    • Cellular localizationCell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • G protein coupled receptor 97 antibody
      • G-protein coupled receptor 97 antibody
      • GPCR 97 antibody
      • GPCR97 antibody
      • H3R antibody
      • HH 3R antibody
      • HH3 R antibody
      • HH3R antibody
      • Histamine H3 receptor antibody
      • Histamine receptor H3 antibody
      • HRH 3 antibody
      • HRH3 antibody
      • HRH3_HUMAN antibody
      • OTTHUMP00000031459 antibody
      see all

    Anti-HRH3 antibody [EPR5631] images

    • All lanes : Anti-HRH3 antibody [EPR5631] (ab124732)

      Lane 1 : Jurkat cell lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : C6 cell lysate
      Lane 4 : A549 cell lysate
      Lane 5 : Neuro-2a cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 49 kDa
    • Flow cytometric analysis of Jurkat cells using ab124732 (red)at 1/100 dilution or a rabbit IgG (negative) (green)
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-HRH3 antibody [EPR5631] (ab124732)

    ab124732 has not yet been referenced specifically in any publications.

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