Overview

  • Product nameAnti-HRH4 antibody
    See all HRH4 primary antibodies
  • Description
    Rabbit polyclonal to HRH4
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 1-52 of Human HRH4 (NP_067637).

  • Positive control
    • 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates This antibody gave a positive result when used in the following formaldehyde fixed cell line: HCT116.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 50% Glycerol, 1X PBS, pH 7.0
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab97487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 44 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionThe H4 subclass of histamine receptors could mediate the histamine signals in peripheral tissues. Displays a significant level of constitutive activity (spontaneous activity in the absence of agonist).
  • Tissue specificityExpressed primarily in the bone marrow and eosinophils. Shows preferential distribution in cells of immunological relevance such as T-cells, dendritic cells, monocytes, mast cells, neutrophils. Also expressed in a wide variety of peripheral tissues, including the heart, kidney, liver, lung, pancreas, skeletal muscle, prostate, small intestine, spleen, testis, colon, fetal liver and lymph node.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AXOR35 antibody
    • BG26 antibody
    • G protein coupled receptor 105 antibody
    • G-protein coupled receptor 105 antibody
    • GPCR105 antibody
    • GPRv53 antibody
    • H4 antibody
    • H4R antibody
    • HH4R antibody
    • Histamine H4 receptor antibody
    • Histamine receptor H4 antibody
    • Hrh4 antibody
    • HRH4_HUMAN antibody
    • Pfi-013 antibody
    • SP9144 antibody
    see all

Anti-HRH4 antibody images

  • ICC/IF image of ab97487 stained HCT116 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab97487 at 10 µg/mL overnight at +4°C. The secondary antibody (green) was DyLight® 488 Goat anti-Rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-HRH4 antibody (ab97487) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : 44 kDa
    10% SDS PAGE

References for Anti-HRH4 antibody (ab97487)

ab97487 has not yet been referenced specifically in any publications.

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J’ai contacté le laboratoire et malheureusement, nous ne vendons pas le peptide correspondant à ab97487. Le laboratoire m’a aussi confirmé que nous n’allons pas l&rs...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"