Overview

  • Product nameAnti-HRH4 antibody
    See all HRH4 primary antibodies
  • Description
    Rabbit polyclonal to HRH4
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Dog, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Mouse HRH4.

    (Peptide available as ab109635.)

  • Positive control
    • This antibody gave a positive signal in Raw264.7 and EL4 whole cell lysates as well as the following tissue lysates: Mouse brain; Mouse bone marrow; Mouse spleen; Mouse thymus.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).

Target

  • FunctionThe H4 subclass of histamine receptors could mediate the histamine signals in peripheral tissues. Displays a significant level of constitutive activity (spontaneous activity in the absence of agonist).
  • Tissue specificityExpressed primarily in the bone marrow and eosinophils. Shows preferential distribution in cells of immunological relevance such as T-cells, dendritic cells, monocytes, mast cells, neutrophils. Also expressed in a wide variety of peripheral tissues, including the heart, kidney, liver, lung, pancreas, skeletal muscle, prostate, small intestine, spleen, testis, colon, fetal liver and lymph node.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AXOR35 antibody
    • BG26 antibody
    • G protein coupled receptor 105 antibody
    • G-protein coupled receptor 105 antibody
    • GPCR105 antibody
    • GPRv53 antibody
    • H4 antibody
    • H4R antibody
    • HH4R antibody
    • Histamine H4 receptor antibody
    • Histamine receptor H4 antibody
    • Hrh4 antibody
    • HRH4_HUMAN antibody
    • Pfi-013 antibody
    • SP9144 antibody
    see all

Anti-HRH4 antibody images

  • All lanes : Anti-HRH4 antibody (ab97997) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Mouse Bone Marrow Tissue Lysate
    Lane 3 : Spleen (Mouse) Tissue Lysate
    Lane 4 : Thymus (Mouse) Tissue Lysate
    Lane 5 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
    Lane 6 : EL4 whole cell lysate (ab7183)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 44 kDa
    Observed band size : 44 kDa
    Additional bands at : 68 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

References for Anti-HRH4 antibody (ab97997)

ab97997 has not yet been referenced specifically in any publications.

Product Wall

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