The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
RelevanceApoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Hrk, a pro-apoptotic member of the Bcl-2 homology domain-3 (BH3) group of the Bcl-2 family of proteins, was identified as a novel protein induced during programmed neuronal death. It lacks significant homology to other Bcl-2 family members except for an 8-amino acid region that is similar to the BH3 motif of Bik. Hrk regulates apoptosis through interaction with the anti-apoptotic proteins Bcl-2 and Bcl-XL via this domain. It does not interact with the pro-apoptotic proteins Bax, Bak, or Bcl-XS. Hrk localizes to mitochondrial membranes in a pattern similar to that previously reported for Bcl-2 and Bcl-XL. Despite its predicted molecular weight, Hrk often migrates at 12-15 kDa.
ab59907, at a 1/50 dilution, staining Hrk in formalin-fixed, paraffin-embedded human breast carcinoma tissue by Immunohistochemistry. The primary antibody was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
References for Anti-Hrk antibody (ab59907)
This product has been referenced in:
Barrera-Vilarmau S et al. Intrinsic order and disorder in the bcl-2 member harakiri: insights into its proapoptotic activity. PLoS One6:e21413 (2011).
Read more (PubMed: 21731739) »
Sborgi L et al. Characterization of a Novel Interaction between Bcl-2 Members Diva and Harakiri. PLoS One5:e15575 (2010).
Read more (PubMed: 21209886) »