Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Peroxidase [Horseradish]. Cross reactivity against Peroxidase from other tissues may occur but have not been specifically determined.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Dot: Use at an assay dependent dilution.
ELISA: 1/1000 - 1/6000. This product has been assayed against 1.0µg of Peroxidase [Horseradish] in a standard sandwich ELISA using Alklaine Phosphatase conjugated affinity purified anti-Rat IgG [H&L] (Goat) and pNPP as a substrate for 30 minutes at RT.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 35 kDa.
Suitable for most immunological methods requiring high titer and specificity.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems.
HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
Secreted Probable. Vacuole Probable. Note: Carboxy-terminal extension appears to target the protein to vacuoles.