Overview

  • Product nameAnti-HRP antibody
    See all HRP primary antibodies
  • Description
    Rat polyclonal to HRP
  • SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Peroxidase [Horseradish]. Cross reactivity against Peroxidase from other tissues may occur but have not been specifically determined.
  • Tested applicationsSuitable for: Dot blot, WB, ELISA, IPmore details
  • Species reactivity
    Reacts with Horseradish.
  • Immunogen

    Full length Horseradish Peroxidase.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Constituents: 0.87% Sodium chloride, 0.164% Sodium phosphate
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by a delipidation and defibrination.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot
WB
ELISA
IP
  • Application notesDot: Use at an assay dependent dilution.
    ELISA: 1/1000 - 1/6000. This product has been assayed against 1.0µg of Peroxidase [Horseradish] in a standard sandwich ELISA using Alklaine Phosphatase conjugated affinity purified anti-Rat IgG [H&L] (Goat) and pNPP as a substrate for 30 minutes at RT.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 35 kDa.

    Suitable for most immunological methods requiring high titer and specificity.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceHorseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
    • Cellular localizationSecreted Probable. Vacuole Probable. Note: Carboxy-terminal extension appears to target the protein to vacuoles.
    • Alternative names
      • Horseradish Peroxidase antibody
      • HPRC1 antibody
      • HRP antibody
      • Peroxidase antibody
      • Peroxidase C1A antibody
      • PRXC1A antibody
      see all

    References for Anti-HRP antibody (ab34827)

    ab34827 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab34827.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"