Overview

  • Product name
  • Description
    Rabbit polyclonal to HRP
  • Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Peroxidase [Horseradish]. Cross reactivity against Peroxidase from other tissues and species may occur but have not been specifically determined.
  • Tested applications
    Suitable for: ELISAmore details
  • Immunogen

    Full length Horseradish peroxidase.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.40
    Constituent: Whole serum
  • Purity
    Whole antiserum
  • Purification notes
    This product was prepared from monospecific antiserum by a delipidation and defibrination process.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000 - 1/20000.

Target

  • Relevance
    Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
  • Cellular localization
    Secreted Probable. Vacuole Probable. Note: Carboxy-terminal extension appears to target the protein to vacuoles.
  • Alternative names
    • Horseradish Peroxidase antibody
    • HPRC1 antibody
    • HRP antibody
    • Peroxidase antibody
    • Peroxidase C1A antibody
    • PRXC1A antibody
    see all

References for Anti-HRP antibody (ab34885)

This product has been referenced in:
  • Xiao C  et al. SPAG9 is overexpressed in osteosarcoma, and regulates cell proliferation and invasion through regulation of JunD. Oncol Lett 12:2674-2679 (2016). IHC . Read more (PubMed: 27698841) »

See 1 Publication for this product

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I have been in contact with the lab regarding this issue and have been informed that this product is not a purified IgG fraction as stated on the datasheet but is instead whole antiserum. This would explain the...

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