Overview

  • Product name
    Anti-HRP antibody - Azide free
    See all HRP primary antibodies
  • Description
    Rat polyclonal to HRP - Azide free
  • Tested applications
    Suitable for: ELISA, Dot blot, WB, IP, Conjugationmore details
  • Species reactivity
    Reacts with Horseradish.
  • Immunogen

    Horseradish peroxidase.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    pH: 7.40
    Constituents: 0.87% Sodium chloride, 0.42% Potassium phosphate
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    This antibody was purified from monospecific antiserum by a multi step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Dot blot
WB
IP
Conjugation
  • Application notes
    Conjugation: Use at an assay dependent dilution.
    Dot blot: Use at an assay dependent dilution.
    ELISA: 1/2000 - 1/10000.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution.
    This antibody is also suitable for most immunological methods requiring high titer and specificity.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
    • Cellular localization
      Secreted Probable. Vacuole Probable. Note: Carboxy-terminal extension appears to target the protein to vacuoles.
    • Alternative names
      • Horseradish Peroxidase antibody
      • HPRC1 antibody
      • HRP antibody
      • Peroxidase antibody
      • Peroxidase C1A antibody
      • PRXC1A antibody
      see all

    References for Anti-HRP antibody - Azide free (ab34694)

    ab34694 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab34694.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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