Overview

  • Product name
    HRP Conjugation Kit
  • Product overview

    HRP Conjugation Kit ab102890 uses a simple and quick process to conjugate an antibody to HRP. It can also be used to conjugate other proteins or peptides.


    To conjugate an antibody to HRP using this kit:
    (check protocol booklet first)
    - add modifier reagent to antibody and incubate for 3 hrs
    - add quencher reagent and incubate for 30 mins
    The conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification steps are required and 100% of the antibody is recovered for use.


    Learn about buffer compatibility below; if your buffer is incompatible or antibody concentration is too low, please use our rapid antibody purification and concentration kits. Use our FAQs to learn more about the technology, or about conjugating other proteins and peptides to HRP.

  • Notes

    Amount and volume of antibody for conjugation to HRP
    (up to the following amounts)

     Kit size   Amount of antibody
    at 4:1 molar ratio1 
    Amount of antibody
    at 1:1 molar ratio1
    Maximum antibody
    volume2
    30 µg   3 x 10 µg  3 x 40 µg 3 x 10 µL
    100 µg   100 µg   400 µg 100 µL
    300 µg   3 x 100 µg  3 x 400 µg 3 x 100 µL
    1 mg   1 mg 4 mg 1 mL

    1 Optimal HRP:antibody molar ratio is 4:1. Antibodies often also perform well at a 1:1 molar ratio.

    2 Ideal antibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 5mg/ml or < 0.5 mg/ml should be diluted /concentrated.

     

    Sample Buffer Requirements

    Compatible buffer constituents
    (up to the concentrations shown, and pH 6.5-8.5):

    50mM Tris 0.1%/1% BSA* 0.1% gelatin 
    0.1% sodium azide** PBS pH6.5-8.5 50% glycerol
    0.15M sodium chloride 50mM HEPES 0.02M potassium phosphate
    0.001% Tween 5% Trehalose EDTA

    * 1% BSA gives lower quality conjugates.
    ** Sodium azide irreversibly inhibits HRP; antibodies with azide should be purified before using the HRP conjugation kit.

    Incompatible buffer constituents
    (in addition to any compatible constituents above the acceptable concentrations):

    Urea  >50mM Imidazole  Glycine
    Ethanolamine DTT Mercaptoethanol
    Thiomerosal Other thiols  Proclin

    Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.

  • Tested applications
    Suitable for: Conjugationmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab102890 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Conjugation Use at an assay dependent dilution.

Images

Protocols

References

This product has been referenced in:
  • Park JK  et al. Evaluation of Preexisting Anti-Hemagglutinin Stalk Antibody as a Correlate of Protection in a Healthy Volunteer Challenge with Influenza A/H1N1pdm Virus. MBio 9:N/A (2018). Read more (PubMed: 29362240) »
  • Joe MK  et al. Myocilin Regulates Metalloprotease 2 Activity Through Interaction With TIMP3. Invest Ophthalmol Vis Sci 58:5308-5318 (2017). Read more (PubMed: 29049729) »

See all 5 Publications for this product

Customer reviews and Q&As

Thank you for contacting us.
As my colleague mentioned, we do not currently carry any antibodies to the Aftose virus. You may be able to find one by searching biocompare.com.
For the other products you mentioned, we do have a number of HRP co...

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Thank you for your inquiry. In case the customer would like to perform a sandwich ELISA (sELISA) for the EBV viral capsid antigen, I would suggest to test the antibody clones [2E08] and [8.F.90] (ab48414 and ab32803, see below). To our knowledge, t...

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Thank you for contacting us. I am sorry to hear you are experiencing difficulties with some of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. In this case the...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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