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Abcam’s HRP Stability Conjugate Reagent (ab171537) was developed specifically for Horseradish Peroxidase (HRP) conjugates in solution. ab171537 is readily incorporated into virtually any assay production protocol. Conjugates can be added directly to ab171537 and are then ready for storage and use. It is also an excellent diluent, and often decreases background and increases signal for improved signal-to-noise ratios. ab171537 prevents the loss of catalytic activity while it maintains the conformation of the antibody or protein-antigen portion of the conjugate.
Recommendations for Use of ab171537 HRP Stability Conjugate Reagent
NOTE – When storing any conjugate/ HRP Stability Conjugate Reagent solution for an extended period of time: If a phosphate buffer is used for dilution we recommend a final concentration of no more than 5 mM buffer to avoid the possibility of initiating precipitation.
Our Abpromise guarantee covers the use of ab171537 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
HRP Stability Conjugate Reagent (ab171537) efficacy was verified by real-time stability studies that included independent testing of the enzyme activity and the immunological activity by ELISA.
The studies were conducted using a monoclonal IgG-HRP conjugate. Results of these analyses indicate superior performance of ab171537 compared to a common HRP conjugate diluent. The monoclonal IgG-HRP conjugate retained over 90% of its activity when stored in HRP Stability Conjugate Reagent for 24 months at room temperature.
ab171537 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"