HRP Stability Conjugate Reagent (ab171537)

Overview

  • Product name
    HRP Stability Conjugate Reagent
    See all ELISA buffer reagents
  • Tested applications
    Suitable for: ELISAmore details
    Unsuitable for: IHC-Fr or IHC-P
  • General notes

    Abcam’s HRP Stability Conjugate Reagent (ab171537) was developed specifically for Horseradish Peroxidase (HRP) conjugates in solution. ab171537 is readily incorporated into virtually any assay production protocol. Conjugates can be added directly to ab171537 and are then ready for storage and use. It is also an excellent diluent, and often decreases background and increases signal for improved signal-to-noise ratios. ab171537 prevents the loss of catalytic activity while it maintains the conformation of the antibody or protein-antigen portion of the conjugate.

    Recommendations for Use of ab171537 HRP Stability Conjugate Reagent

    • For optimal stability and performance, use ab171537 at 100% concentration
    • Dilute your HRP conjugate to optimum working concentration in the HRP Stability Conjugate Reagent solution
    • Use diluted conjugate working solution in your assay as normal
    • Or, preferably, store away from direct light at room temperature and use as a stock solution for optimum assay-to-assay consistency

    NOTE – When storing any conjugate/ HRP Stability Conjugate Reagent solution for an extended period of time: If a phosphate buffer is used for dilution we recommend a final concentration of no more than 5 mM buffer to avoid the possibility of initiating precipitation.

Properties

  • Form
    Liquid
  • Storage instructions
    Store at room temperature.
  • Storage buffer
    Preservatives: 0.002% Proclin, 0.02% 5-Bromo-5-nitro-1,3-dioxane
    Constituents: 98% Proprietary component, 0.02% 2-methyl-4-isothiazolin-3-one
    Note: pH 6.2 - 6.7. Contains BSA
  • Concentration information loading...

Applications

Our Abpromise guarantee covers the use of ab171537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-Fr or IHC-P.
  • Images

    • HRP Stability Conjugate Reagent (ab171537) efficacy was verified by real-time stability studies that included independent testing of the enzyme activity and the immunological activity by ELISA.

      The studies were conducted using a monoclonal IgG-HRP conjugate. Results of these analyses indicate superior performance of ab171537 compared to a common HRP conjugate diluent. The monoclonal IgG-HRP conjugate retained over 90% of its activity when stored in HRP Stability Conjugate Reagent for 24 months at room temperature.

    References

    ab171537 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    We use a 1:10,000 dilution of the conjugate in ab171537. We recommend you first try diluting the HRP conjugate to your normal use concentration. You may consider a small accelerated study to determine whether or not you are getting the stability you need.

    Yes.

    We recommend using ab171537 undiluted for maximum stabilization. However, in cases where you feel it may be necessary to dilute the products (e.g., 1:1) please use ab171537 diluted in deionized water.
    NOTE: Phosphate buffers cause precipitation in ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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