Overview

  • Product name
  • Description
    Rabbit polyclonal to HRPT2
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residues 250 and 300 of human HRPT2.

  • Positive control
    • Whole cell lysate from control 293T cells or 293T cells transfected with a HRPT2 expression construct.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7.0-8.0
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab70533 was affinity purified using an epitope specific to HRPT2 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at 1 - 4 µg/mg of lysate.
    WB: 1/5,000 - 1/20,000. Detects a band of approximately 64 kDa (predicted molecular weight: 61 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Tumor suppressor probably involved in transcriptional and post-transcriptional control pathways. May be involved in cell cycle progression through the regulation of cyclin D1/PRAD1 expression. Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of MLL1; it promotes leukemogenesis though association with MLL-rearranged oncoproteins, such as MLL-MLLT3/AF9 and MLL-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Connects PAF1C with the cleavage and polyadenylation specificity factor (CPSF) complex and the cleavage stimulation factor (CSTF) complex, and with Wnt signaling. Involved in polyadenylation of mRNA precursors.
    • Tissue specificity
      Found in adrenal and parathyroid glands, kidney and heart.
    • Involvement in disease
      Defects in CDC73 are a cause of familial isolated hyperparathyroidism (FIHP) [MIM:145000]; also known as hyperparathyroidism type 1 (HRPT1). FIHP is an autosomal dominant disorder characterized by hypercalcemia, elevated parathyroid hormone (PTH) levels, and uniglandular or multiglandular parathyroid tumors.
      Defects in CDC73 are the cause of hyperparathyroidism-jaw tumor syndrome (HPT-JT) [MIM:145001]; also known as hyperparathyroidism type 2 (HRPT2) or familial primary hyperparathyroidism with multiple ossifying jaw fibromas. HPT-JT is an autosomal dominant, multiple neoplasia syndrome primarily characterized by hyperparathyroidism due to parathyroid tumors. Thirty percent of individuals with HPT-JT may also develop ossifying fibromas, primarily of the mandible and maxilla, which are distinc from the brown tumors associated with severe hyperparathyroidism. Kidney lesions may also occur in HPT-JT as bilateral cysts, renal hamartomas or Wilms tumors.
      Defects in CDC73 are a cause of parathyroid carcinoma (PRTC) [MIM:608266]. These cancers characteristically result in more profound clinical manifestations of hyperparathyroidism than do parathyroid adenomas, the most frequent cause of primary hyperparathyroidism. Early en bloc resection of the primary tumor is the only curative treatment.
    • Sequence similarities
      Belongs to the CDC73 family.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • C1orf28 antibody
      • CDC 73 antibody
      • Cdc73 antibody
      • CDC73_HUMAN antibody
      • Cell division cycle 73 antibody
      • Cell division cycle 73 Paf1/RNA polymerase II complex component homolog antibody
      • Cell division cycle 73 Paf1/RNA polymerase II complex component like protein antibody
      • Cell division cycle protein 73 homolog antibody
      • FIHP antibody
      • FLJ23316 antibody
      • HPT JT antibody
      • HPTJT antibody
      • HRPT 2 antibody
      • HRPT1 antibody
      • HRPT2 antibody
      • Hyperparathyroidism 2 (with jaw tumor) antibody
      • Hyperparathyroidism 2 antibody
      • Hyperparathyroidism 2 protein antibody
      • HYX antibody
      • Paf1/RNA polymerase II complex component antibody
      • Parafibromin antibody
      see all

    Images

    • All lanes : Anti-HRPT2 antibody (ab70533) at 0.1 µg/ml

      Lane 1 : Whole cell lysate from 293T cells at 10 µg
      Lane 2 : Whole cell lysate from 293T cells at 20 µg
      Lane 3 : Whole cell lysate from 293T cells transfected with a HRPT2 expression construct at 10 µg

      Developed using the ECL technique

      Predicted band size : 61 kDa
      Observed band size : 64 kDa (why is the actual band size different from the predicted?)


      Exposure time : 5 minutes
    • Detection of Human HRPT2 (approximately 64 kDa) by Immunoprecipitation in whole cell lysate from 293T cells (10 mg for IP) in the presence or absence of blocking peptide, using ab70533 (BL648) at 3 µg/10mg lysate for IP and at 0.2 µg/ml for subsequent WB detection.

    References

    ab70533 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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