Anti-HS3ST3B1 antibody (ab105322)

Overview

  • Product nameAnti-HS3ST3B1 antibody
  • Description
    Rabbit polyclonal to HS3ST3B1
  • Tested applicationsSuitable for: ICC, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide: AMLCVWLYMF LYSCAGSCAA APGLLLLGSG SRAAHDPPAL ATAPDGTPPR, corresponding to a region within internal sequence amino acids 38-87 of HS3ST3B1

  • Positive control
    • Human fetal muscle lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab105322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionSulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to an N-unsubstituted glucosamine linked to a 2-O-sulfo iduronic acid unit on heparan sulfate. Catalyzes the O-sulfation of glucosamine in IdoUA2S-GlcNS and also in IdoUA2S-GlcNH2. The substrate-specific O-sulfation generates an enzyme-modified heparan sulfate which acts as a binding receptor to Herpes simplex virus-1 (HSV-1) and permits its entry. Unlike 3-OST-1, does not convert non-anticoagulant heparan sulfate to anticoagulant heparan sulfate.
  • Tissue specificityUbiquitous. Most abundant in liver and placenta, followed by heart and kidney.
  • Sequence similaritiesBelongs to the sulfotransferase 1 family.
  • Cellular localizationGolgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3-OST-3B antibody
    • h3-OST-3B antibody
    • Heparan sulfate 3-O-sulfotransferase 3B1 antibody
    • Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 3B1 antibody
    • Heparan sulfate glucosamine 3-O-sulfotransferase 3B1 antibody
    • HS3SB_HUMAN antibody
    • HS3ST3B1 antibody
    see all

Anti-HS3ST3B1 antibody images

  • Anti-HS3ST3B1 antibody (ab105322) at 1 µg/ml + Human fetal muscle lysate at 10 µg

    Predicted band size : 43 kDa

References for Anti-HS3ST3B1 antibody (ab105322)

ab105322 has not yet been referenced specifically in any publications.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cultured Cells (RWPE-1)
Specification RWPE-1
Fixative Methanol
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 30°C
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Submitted Aug 23 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"