Overview

  • Product nameAnti-HS3ST6 antibody
  • Description
    Rabbit polyclonal to HS3ST6
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Chimpanzee, Monkey, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 217-266 (VSGERLVSDP AGEVGRVQDF LGLKRVVTDK HFYFNATKGF PCLKKAQGGS) of human HS3ST6 (NP_001009606).

  • Positive control
    • 721_B cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab81036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 37 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/12500.

Target

  • RelevanceHS3ST6 is a single-pass type II membrane protein. It belongs to the sulfotransferase 1 family. It transfers a sulfuryl group to heparan sulfate. The substrate-specific O-sulfation generates an enzyme-modified heparan sulfate which acts as a binding receptor to Herpes Simplex Virus-1 (HSV-1) and permits its entry. Unlike 3-OST-1, does not convert non-anticoagulant heparan sulfate to anticoagulant heparan sulfate.
  • Cellular localizationGolgi apparatus membrane; Single-pass type II membrane protein.
  • Database links
  • Alternative names
    • h3 OST 6 antibody
    • Heparan sulfate (glucosamine) 3 O sulfotransferase 5 antibody
    • Heparan sulfate (glucosamine) 3 O sulfotransferase 6 antibody
    • Heparan sulfate 3 O sulfotransferase 6 antibody
    • Heparan sulfate D glucosaminyl 3 O sulfotransferase 6 antibody
    • Heparan sulfate glucosamine 3 O sulfotransferase 6 antibody
    • Heparan sulphate D glucosaminyl 3 O sulfotransferase 3B like antibody
    • HS3ST5 antibody
    see all

Anti-HS3ST6 antibody images

  • Anti-HS3ST6 antibody (ab81036) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 37 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands.

References for Anti-HS3ST6 antibody (ab81036)

ab81036 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"