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Rabbit polyclonal to HS6ST2
antigen sequence: RKRQEQRKFL KGRLLQTHFQ SQGQGQSQNP NQNQSQNPNP NANQNLTQNL MQNLTQSLSQ KENRESPKQN SGKEQNDNTS NGTND, corresponding to amino acids 512-596 of Human HS6ST2.
- Human cerebral cortex tissue; RT-4 and U-251 MG lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
6-O-sulfation enzyme which catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 6 of the N-sulfoglucosamine residue (GlcNS) of heparan sulfate.
Belongs to the sulfotransferase 6 family.
Information by UniProt
- H6ST2_HUMAN antibody
- Heparan-sulfate 6-O-sulfotransferase 2 antibody
- HS6ST-2 antibody
Immunocytochemistry/ Immunofluorescence - Anti-HS6ST2 antibody (ab122220)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab122220 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HS6ST2 antibody (ab122220)
ab122220, at 1/125 dilution, staining HS6ST2 in paraffin-embedded Human Cerebral cortex tissue by Immunohistochemistry.
Western blot - Anti-HS6ST2 antibody (ab122220)
All lanes : Anti-HS6ST2 antibody (ab122220) at 1/250 dilution
Lane 1 : RT-4 lysate
Lane 2 : U-251 MG lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"