Overview

  • Product name
    Anti-HS6ST2 antibody
  • Description
    Rabbit polyclonal to HS6ST2
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: RKRQEQRKFL KGRLLQTHFQ SQGQGQSQNP NQNQSQNPNP NANQNLTQNL MQNLTQSLSQ KENRESPKQN SGKEQNDNTS NGTND, corresponding to amino acids 512-596 of Human HS6ST2.

  • Positive control
    • Human cerebral cortex tissue; RT-4 and U-251 MG lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab122220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • Function
    6-O-sulfation enzyme which catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 6 of the N-sulfoglucosamine residue (GlcNS) of heparan sulfate.
  • Sequence similarities
    Belongs to the sulfotransferase 6 family.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • H6ST2_HUMAN antibody
    • Heparan-sulfate 6-O-sulfotransferase 2 antibody
    • HS6ST-2 antibody
    • HS6ST2 antibody
    • RP3-435D1.3 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab122220 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122220, at 1/125 dilution, staining HS6ST2 in paraffin-embedded Human Cerebral cortex tissue by Immunohistochemistry.
  • All lanes : Anti-HS6ST2 antibody (ab122220) at 1/250 dilution

    Lane 1 : RT-4 lysate
    Lane 2 : U-251 MG lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique

References

ab122220 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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