Overview

  • Product nameAnti-HS6ST3 antibody
  • Description
    Rabbit polyclonal to HS6ST3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide designed within residues TKQLEHQRDR QKRREERRLQ REHRDHQWPK EDGAAEGTVT EDYNSQVVRW, corresponding to C terminal amino acids 422-471 of Human HS6ST3 (NP_703157)

  • Positive control
    • Fetal brain lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 55 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceHS6ST3 (Heparan-sulphate 6-O-sulfotransferase 1) is a single-pass type II transmembrane protein that catalyses the transfer of sulphate from PAPS (3'-phosphoadenosine 5'-phosphosulphate) to position 6 of the N-sulphoglucosamine residue of heparan sulphate. Heparan sulfate (HS) sulfotransferases, such as HS6ST3, modify HS to generate structures required for interactions between HS and a variety of proteins. These interactions are implicated in proliferation and differentiation, adhesion, migration, inflammation, blood coagulation, and other diverse processes.
  • Cellular localizationMembrane; Single-pass type II membrane protein
  • Database links
  • Alternative names
    • 6OST3 antibody
    • DKFZp761K2315 antibody
    • Heparan sulfate 6-O-sulfotransferase 3 antibody
    • HS6ST 3 antibody
    • MGC144859 antibody
    • MGC144860 antibody
    see all

Anti-HS6ST3 antibody images

  • Anti-HS6ST3 antibody (ab82719) at 1 µg/ml + fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 55 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)

References for Anti-HS6ST3 antibody (ab82719)

ab82719 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Kidney)
Specification Kidney
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TIER pH6 for 10 min. cooling down for 2 hrs in buffer
Permeabilization No
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Jul 29 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"