HsAFM ChIP primer Exon 1 (Forward) (ab83606)

Overview

  • Product name
    HsAFM ChIP primer Exon 1 (Forward)
    See all AFM dna rna and cdna
  • General notes

    Accession number: NM_001133.The HsAFM primers and probe can be used to quantify a control locus in chromatin immunoprecipitation (ChIP) assays with chromatin prepared from human cells. AFM (Alpha-albumin) is expressed in liver but inactive in most other cell types. The primers and probe are located in exon 1. In most cell types AFM is enriched with histone modifications associated with silencing (e.g. Histone H3 K27 di methylation, see image with ab24684). Whereas it shows low levels of histone modifications associated with active gene transcription such as Histone H3 K18 acetylation (see image with ab1191).

  • Tested applications
    Suitable for: Real Time PCRmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab83606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Real Time PCR Use at an assay dependent dilution. Suitable for the analysis of chromatin immunoprecipitated DNA using Taqman® real time PCR. Quantity provided for 200 reactions. Please see the protocol page for the instructions for this product.

References

ab83606 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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