Accession number: NM_001133. The HsAFM primers and probe can be used to quantify a control locus in chromatin immunoprecipitation (ChIP) assays with chromatin prepared from human cells.
AFM (Alpha-albumin) is expressed in liver but inactive in most other cell types. The primers and probe are located in exon 1. In most cell types AFM is enriched with histone modifications associated with silencing (e.g. Histone H3 K27 di methylation, see image with ab24684). Whereas it shows low levels of histone modifications associated with active gene transcription such as Histone H3 K18 acetylation (see image with ab1191).
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Real Time PCR
Use at an assay dependent dilution. Suitable for the analysis of chromatin immunoprecipitated DNA using Taqman® real time PCR. The probe is labelled with FAM/TAMRA.
Quantity provided for 200 reactions. Please see the protocol page for the instructions for this product.