Accession number: NM_184043.The HsALDOA primers and probe can be can be used to quantify a control locus in chromatin immunoprecipitation (ChIP) assays with chromatin prepared from human cells. ALDOA (Fructose-bisphosphate aldolase A) is a moderately expressed housekeeping gene. The primers and probe are located in intron 2, within 1 kb of the transcription start site. In most cell types, this locus is enriched with histone modifications associated with active gene transcription such as Histone H3 K9 acetylation (see image with ab4441). Whereas it shows low levels of histone modifications associated with silencing such as Histone H3 K9 tri methylation (see image with ab8898).
Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein.
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
Involvement in disease
Defects in ALDOA are the cause of glycogen storage disease type 12 (GSD12) [MIM:611881]; also known as red cell aldolase deficiency. A metabolic disorder associated with increased hepatic glycogen and hemolytic anemia. It may lead to myopathy with exercise intolerance and rhabdomyolysis.
Belongs to the class I fructose-bisphosphate aldolase family.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Real Time PCR
Use at an assay dependent dilution. Suitable for the analysis of chromatin immunoprecipitated DNA using Taqman® real time PCR.
Quantity provided for 200 reactions. Please see the protocol page for the instructions for this product.