HsAldolase ChIP primer Intron 2 (Forward) (ab83591)

Overview

  • Product name
    HsAldolase ChIP primer Intron 2 (Forward)
    See all Aldolase dna rna and cdna
  • General notes

    Accession number: NM_184043.The HsALDOA primers and probe can be can be used to quantify a control locus in chromatin immunoprecipitation (ChIP) assays with chromatin prepared from human cells. ALDOA (Fructose-bisphosphate aldolase A) is a moderately expressed housekeeping gene. The primers and probe are located in intron 2, within 1 kb of the transcription start site. In most cell types, this locus is enriched with histone modifications associated with active gene transcription such as Histone H3 K9 acetylation (see image with ab4441). Whereas it shows low levels of histone modifications associated with silencing such as Histone H3 K9 tri methylation (see image with ab8898).

  • Tested applications
    Suitable for: Real Time PCRmore details

Properties

  • Function
    Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein.
  • Pathway
    Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
  • Involvement in disease
    Defects in ALDOA are the cause of glycogen storage disease type 12 (GSD12) [MIM:611881]; also known as red cell aldolase deficiency. A metabolic disorder associated with increased hepatic glycogen and hemolytic anemia. It may lead to myopathy with exercise intolerance and rhabdomyolysis.
  • Sequence similarities
    Belongs to the class I fructose-bisphosphate aldolase family.
  • Information by UniProt
  • Alternative names
    • ALDA
    • Aldo1
    • ALDOA
    • ALDOA_HUMAN
    • Aldolase 1
    • Aldolase A
    • Aldolase A fructose bisphosphatase
    • Aldolase A fructose bisphosphate
    • Aldolase, fructose-bisphosphate A
    • Epididymis secretory sperm binding protein Li 87p
    • FRUCTOALDOLASE A
    • Fructose 1 6 bisphosphate triosephosphate lyase
    • Fructose bisphosphate aldolase
    • Fructose bisphosphate aldolase A
    • FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE A
    • Fructose-bisphosphate aldolase A
    • Fructose-bisphosphate aldolase A Muscle-type
    • GSD12
    • HEL S 87p
    • Lung cancer antigen NY LU 1
    • Lung cancer antigen NY-LU-1
    • MGC10942
    • MGC17716
    • MGC17767
    • Muscle type aldolase
    • Muscle-type aldolase
    • RNALDOG5
    see all

Applications

Our Abpromise guarantee covers the use of ab83591 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Real Time PCR Use at an assay dependent dilution. Suitable for the analysis of chromatin immunoprecipitated DNA using Taqman® real time PCR. Quantity provided for 200 reactions. Please see the protocol page for the instructions for this product.

References for HsAldolase ChIP primer Intron 2 (Forward) (ab83591)

ab83591 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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