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Full length protein corresponding to Mouse Hsc70. Mouse spermatogenic cell protein
Our Abpromise guarantee covers the use of ab2788 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/20 - 1/200.|
|Inhibition Assay||Use at an assay dependent concentration.|
|IHC-Fr||1/20 - 1/200.|
|IP||Use at an assay dependent concentration.
Suggest 2 μl
|WB||1/1000 - 1/5000. Detects a band of approximately 70 kDa.|
Immunocytochemistry/Immunofluorescence analysis of Hsc70 (green) in HeLa and NIH3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% BSA for 15 minutes at room temperature. Cells were incubated with ab2788 (1:50) for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488 goat anti-mouse IgG secondary antibody (1:400) for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification.
Western blot analysis of Hsc70 was performed by loading 50µg of the indicated whole cell lysates and 15µl of prestained protein ladder onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was incubated with ab2788 (1:1000) overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween 20, and incubated with a goat anti-mouse IgM-HRP secondary antibody (1:20,000) for at least 1 hour. Chemiluminescent detection was performed.
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