Overview

  • Product nameAnti-Hsc70 [5B7] antibodySee all Hsc70 primary antibodies ...
  • Description
    Mouse monoclonal [5B7] to Hsc70
  • Specificityab69559 detects an ~70-75 kDa protein, corresponding to the apparent molecular mass of plant cytosolic Hsc70 on SDS-PAGE immunoblots. It does not cross react with E. coli DnaK, mammalian Hsc70, recombinant hamster BiP or spinach BiP.
  • Tested applicationsWB, IP more details
  • Species reactivity
    Reacts with: Plants
  • Immunogen

    A preparation of spinach Hsc70 proteins comprised of cytosolic Hsc70, ER luminal Hsc70 (also known as BiP) and possibly other members of the Hsc70 family of proteins.

  • General notesStorage below -20°C is not recommended.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • Clonality Monoclonal
  • Clone number5B7
  • IsotypeIgG1
  • Light chain typekappa
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab69559 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 70 kDa. Note: Although this antibody works well for immunoprecipitations under native conditions, it is not recommended for immunoprecipitating cytosolic Hsc70 from tomato lysates.
IP Use a concentration of 20 µg/ml.

Target

  • FunctionActs as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the heat shock protein 70 family.
  • DomainThe N-terminal 1-386 residues constitute the ATPase domain, while residues 387-646 form the peptide-binding domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    ISGylated.
  • Cellular localizationCytoplasm. Melanosome. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession P11142 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names
    • 2410008N15Rik antibody
    • Constitutive heat shock protein 70 antibody
    • Heat shock 70 kDa protein 8 antibody
    • Heat shock 70kD protein 10 antibody
    • Heat shock 70kD protein 8 antibody
    • Heat shock 70kDa protein 8 antibody
    • Heat shock cognate 71 kDa protein antibody
    • Heat shock cognate protein 54 antibody
    • Heat shock cognate protein 71 kDa antibody
    • Heat shock protein 8 antibody
    • Heat shock protein A8 antibody
    • Heat-shock70-KD protein 10, formerly antibody
    • HSC54 antibody
    • HSC71 antibody
    • Hsc73 antibody
    • HSP71 antibody
    • HSP73 antibody
    • HSP7C_HUMAN antibody
    • HSPA10 antibody
    • HSPA8 antibody
    • LAP1 antibody
    • Lipopolysaccharide associated protein 1 antibody
    • LPS associated protein 1 antibody
    • LPS associated protein antibody
    • MGC102007 antibody
    • MGC106514 antibody
    • MGC114311 antibody
    • MGC118485 antibody
    • MGC131511 antibody
    • MGC29929 antibody
    • N-myristoyltransferase inhibitor protein 71 antibody
    • NIP71 antibody
    see all

Anti-Hsc70 [5B7] antibody images

  • All lanes : Anti-Hsc70 [5B7] antibody (ab69559) at 1000 µg/ml

    Lane 1 : Molecular weight
    Lane 2 : DnaK Recombinant E. coli Protein
    Lane 3 : Banana Lysate
    Lane 4 : Orange Lysate
    Lane 5 : Carrot Lysate
    Lane 6 : Cytosolic wheat Hsc70


    Predicted band size : 70 kDa
    Observed band size : 70 kDa

References for Anti-Hsc70 [5B7] antibody (ab69559)

ab69559 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"