The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/1 - 1/1000.
FunctionOne HIP oligomer binds the ATPase domains of at least two HSC70 molecules dependent on activation of the HSC70 ATPase by HSP40. Stabilizes the ADP state of HSC70 that has a high affinity for substrate protein. Through its own chaperone activity, it may contribute to the interaction of HSC70 with various target proteins.
Sequence similaritiesBelongs to the FAM10 family. Contains 1 STI1 domain. Contains 3 TPR repeats.
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Anti-HSC70 Interacting Protein HIP antibody images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSC70 Interacting Protein HIP antibody (ab117109)
ab117109 at 10µg/ml staining HSC70 Interacting Protein HIP in Formalin-fixed, Paraffin-embedded Human Adrenal tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
References for Anti-HSC70 Interacting Protein HIP antibody (ab117109)
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