The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Detects a band of approximately 58 kDa (predicted molecular weight: 42 kDa). for human and bovine or 1/5000 for mouse and rat.
FunctionOne HIP oligomer binds the ATPase domains of at least two HSC70 molecules dependent on activation of the HSC70 ATPase by HSP40. Stabilizes the ADP state of HSC70 that has a high affinity for substrate protein. Through its own chaperone activity, it may contribute to the interaction of HSC70 with various target proteins.
Sequence similaritiesBelongs to the FAM10 family. Contains 1 STI1 domain. Contains 3 TPR repeats.