Anti-HSC70 Interacting Protein HIP antibody [EP3264] (ab76005)

Overview

  • Product nameAnti-HSC70 Interacting Protein HIP antibody [EP3264]
    See all HSC70 Interacting Protein HIP primary antibodies
  • Description
    Rabbit monoclonal [EP3264] to HSC70 Interacting Protein HIP
  • Tested applicationsSuitable for: WB, IHC-P, ICC, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A synthetic peptide corresponding to residues near the C terminus of human HSC70 Interacting Protein HIP.

  • Positive control
    • 293T and SW480 cell lysates; human colonic adenocarcinoma and normal colon tissues.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEP3264
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2500 - 1/5000. Predicted molecular weight: 41 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/50 - 1/100.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notesIs unsuitable for IP.
  • Target

    • FunctionOne HIP oligomer binds the ATPase domains of at least two HSC70 molecules dependent on activation of the HSC70 ATPase by HSP40. Stabilizes the ADP state of HSC70 that has a high affinity for substrate protein. Through its own chaperone activity, it may contribute to the interaction of HSC70 with various target proteins.
    • Sequence similaritiesBelongs to the FAM10 family.
      Contains 1 STI1 domain.
      Contains 3 TPR repeats.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • AAG 2 antibody
      • AAG2 antibody
      • Aging associated protein 2 antibody
      • F10A1_HUMAN antibody
      • FAM10A1 antibody
      • FAM10A4 antibody
      • FLJ27260 antibody
      • Heat shock 70kD protein binding protein antibody
      • Hip antibody
      • HOP antibody
      • Hsc70 interacting protein antibody
      • Hsc70-interacting protein antibody
      • Hsp70 interacting protein antibody
      • HSPABP 1 antibody
      • HSPABP antibody
      • HSPABP1 antibody
      • MGC129952 antibody
      • OTTHUMP00000028873 antibody
      • P48 antibody
      • PRO0786 antibody
      • Progesterone receptor associated p48 protein antibody
      • Progesterone receptor-associated p48 protein antibody
      • Protein FAM10A1 antibody
      • Putative tumor suppressor ST13 antibody
      • Renal carcinoma antigen NY REN 33 antibody
      • Renal carcinoma antigen NY-REN-33 antibody
      • SNC 6 antibody
      • SNC6 antibody
      • ST 13 antibody
      • St13 antibody
      • Suppression of tumorigenicity 13 antibody
      • Suppression of tumorigenicity 13 (colon carcinoma) antibody
      • Suppression of tumorigenicity 13 protein antibody
      • Suppression of tumorigenicity protein 13 antibody
      see all

    Anti-HSC70 Interacting Protein HIP antibody [EP3264] images

    • All lanes : Anti-HSC70 Interacting Protein HIP antibody [EP3264] (ab76005) at 1/100000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : SW480 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/1000 dilution
      Developed using the ECL technique

      Predicted band size : 41 kDa
      Observed band size : 47 kDa (why is the actual band size different from the predicted?)
    • ab76005 at 1/100 dilution staining HSC70 Interacting Protein HIP in human colonic adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue.
    • ab76005 at 1/100 dilution staining HSC70 Interacting Protein HIP in human normal colon by Immunohistochemistry, Paraffin-embedded tissue.

    References for Anti-HSC70 Interacting Protein HIP antibody [EP3264] (ab76005)

    ab76005 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab76005.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"