Overview

  • Product name
    Anti-Hsc82 antibody
  • Description
    Rabbit polyclonal to Hsc82
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 650 to the C-terminus of Saccharomyces cerevisiae Hsc82.

    (Peptide available as ab31800.)

  • Positive control
    • Saccharomyces cerevisiae Whole Cell Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab30920 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).

Target

  • Relevance
    The budding yeast Saccharomyces cerevisiae has two genes, HSP82 and HSC82, that encode homologues of HSP90. HSC82 is a molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved in cell cycle control. Cells overexpressing Hsc82 are hypersensitive to various stresses including high concentrations of NaCl. The phenotypes associated with the sensitivity to salt stress of Hsc82-overexpressing cells are similar to those of calcineurin-defective mutants.
  • Cellular localization
    Cytoplasmic and Mitochondrial
  • Database links
    • Alternative names
      • 82 kDa heat shock cognate protein antibody
      • ATP-dependent molecular chaperone HSC82 antibody
      • Heat shock protein Hsp90 constitutive isoform antibody

    Images

    • Anti-Hsc82 antibody (ab30920) at 1 µg/ml + Saccharomyces cerevisiae Whole Cell Lysate at 20 µg

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

      Performed under reducing conditions.

      Predicted band size : 81 kDa
      Observed band size : 81 kDa

    References

    This product has been referenced in:
    • Ly N & Cyert MS Calcineurin, the Ca(2+)-dependent phosphatase, regulates Rga2, a Cdc42 GTPase-activating protein, to modulate pheromone signaling. Mol Biol Cell 28:576-586 (2017). WB ; Saccharomyces cerevisiae . Read more (PubMed: 28077617) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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