Overview

  • Product name
    Anti-HSCB antibody
  • Description
    Rabbit polyclonal to HSCB
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen, corresponding to amino acids 160-232 (QFLIEIMEIN EKLAEAESEA AMKEIESIVK AKQKEFTDNV SSAFEQDDFE EAKEILTKMR YFSNIEEKIK LKK) of Human HSCB.

  • Positive control
    • Human colon tissue; Transfected HEK293T Over-expression Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab121569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/2500.

Target

  • Function
    May act as a co-chaperone in iron-sulfur cluster assembly in mitochondria.
  • Tissue specificity
    Liver, muscle and heart.
  • Pathway
    Cofactor biosynthesis; iron-sulfur cluster biosynthesis.
  • Sequence similarities
    Belongs to the hscB family.
    Contains 1 J domain.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI325508 antibody
    • AW049829 antibody
    • dJ366L4.2 antibody
    • DnaJ (Hsp40) homolog, subfamily C member 20 antibody
    • DnaJ homolog subfamily C member 20 antibody
    • DNAJC20 antibody
    • Hsc20 antibody
    • HSC20_HUMAN antibody
    • hscB antibody
    • HscB iron sulfur cluster co chaperone homolog antibody
    • Iron sulfur cluster co chaperone protein HscB mitochondrial antibody
    • Iron-sulfur cluster co-chaperone protein HscB antibody
    • J type co chaperone HSC20 antibody
    • JAC1 antibody
    • mitochondrial antibody
    • RGD1311005 antibody
    • RP3-366L4.2 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in plasma membrane, cytoplasm and centrosome. Recommended concentration of ab121569 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121569 at 1/450 dilution staining HSCB in Paraffin-embedded Human Colon tissue by Immunohistochemistry.
  • All lanes : Anti-HSCB antibody (ab121569) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)
    Lane 2 : HSCB transfected HEK293T Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))

    Developed using the ECL technique

References

ab121569 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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