Overview

  • Product name
  • Description
    Rabbit polyclonal to HSD11B1
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Fr, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Dog, Pig, Cynomolgus monkey
  • Immunogen

    Synthetic peptide:

    DDIHHVRKSM EVNFL

    , corresponding to internal sequence amino acids 131-145 of Human HSD11B1.

  • Positive control
    • HeLa cell lysate, SMMC cell lysate, Raji cell lysate, Colo320 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab109554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa.
IHC-P Use a concentration of 1 µg/ml.
IHC-Fr Use a concentration of 1 µg/ml.
ICC Use a concentration of 1 µg/ml.

Target

  • Function
    Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7-ketocholesterol to 7-beta-hydroxycholesterol.
  • Tissue specificity
    Widely expressed. Highest expression in liver.
  • Involvement in disease
    Defects in HSD11B1 are a cause of cortisone reductase deficiency (CRD) [MIM:604931]. In CRD, activation of cortisone to cortisol does not occur, resulting in adrenocorticotropin-mediated androgen excess and a phenotype resembling polycystic ovary syndrome (PCOS).
  • Sequence similarities
    Belongs to the short-chain dehydrogenases/reductases (SDR) family.
  • Post-translational
    modifications
    Glycosylated.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 11 beta HSD 1 antibody
    • 11 beta HSD1 antibody
    • 11 beta hydroxysteroid dehydrogenase 1 antibody
    • 11 DH antibody
    • 11-beta hydroxysteroid dehydrogenase, type 1 antibody
    • 11-beta-HSD1 antibody
    • 11-beta-hydroxysteroid dehydrogenase 1 antibody
    • 11-DH antibody
    • 11DH antibody
    • Corticosteroid 11 beta dehydrogenase isozyme 1 antibody
    • Corticosteroid 11-beta-dehydrogenase isozyme 1 antibody
    • CORTRD2 antibody
    • DHI1_HUMAN antibody
    • HDL antibody
    • HSD 11 antibody
    • HSD11 antibody
    • HSD11B antibody
    • HSD11B1 antibody
    • HSD11L antibody
    • Hydroxysteroid (11 beta) dehydrogenase antibody
    • Hydroxysteroid (11 beta) dehydrogenase 1 antibody
    • MGC13539 antibody
    • SDR26C1 antibody
    • short chain dehydrogenase/reductase family 26C, member 1 antibody
    see all

Images

  • All lanes : Anti-HSD11B1 antibody (ab109554) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : SMMC whole cell lysate
    Lane 3 : Raji whole cell lysate
    Lane 4 : Colo320 whole cell lysate


    Predicted band size : 32 kDa

References

This product has been referenced in:
  • Hu Y  et al. In ovo injection of betaine alleviates corticosterone-induced fatty liver in chickens through epigenetic modifications. Sci Rep 7:40251 (2017). WB . Read more (PubMed: 28059170) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HK2 cell (human kidney proximal tubule))
Loading amount
30 µg
Specification
HK2 cell (human kidney proximal tubule)
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7% · Temperature: 20°C
Username

Mr. Dongil Kim

Verified customer

Submitted May 03 2013

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry for any inconvenience that this product has caused. As requested, I have issued a free of char...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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