Overview

  • Product nameAnti-HSD11B1 antibody
    See all HSD11B1 primary antibodies
  • Description
    Rabbit polyclonal to HSD11B1
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human, Pig
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide derived from within N terminal amino acids 72-121 (QKVVSHCLEL GAASAHYIAG TMEDMTFAEQ FVAQAGKLMG GLDMLILNHI), of Human HSD11B1 (NP_005516)

  • Positive control
    • Fetal liver lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab83522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa.Can be blocked with Human HSD11B1 peptide (ab99223). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionCatalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7-ketocholesterol to 7-beta-hydroxycholesterol.
  • Tissue specificityWidely expressed. Highest expression in liver.
  • Involvement in diseaseDefects in HSD11B1 are a cause of cortisone reductase deficiency (CRD) [MIM:604931]. In CRD, activation of cortisone to cortisol does not occur, resulting in adrenocorticotropin-mediated androgen excess and a phenotype resembling polycystic ovary syndrome (PCOS).
  • Sequence similaritiesBelongs to the short-chain dehydrogenases/reductases (SDR) family.
  • Post-translational
    modifications
    Glycosylated.
  • Cellular localizationEndoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 11 beta HSD 1 antibody
    • 11 beta HSD1 antibody
    • 11 beta hydroxysteroid dehydrogenase 1 antibody
    • 11 DH antibody
    • 11-beta hydroxysteroid dehydrogenase, type 1 antibody
    • 11-beta-HSD1 antibody
    • 11-beta-hydroxysteroid dehydrogenase 1 antibody
    • 11-DH antibody
    • 11DH antibody
    • Corticosteroid 11 beta dehydrogenase isozyme 1 antibody
    • Corticosteroid 11-beta-dehydrogenase isozyme 1 antibody
    • CORTRD2 antibody
    • DHI1_HUMAN antibody
    • HDL antibody
    • HSD 11 antibody
    • HSD11 antibody
    • HSD11B antibody
    • HSD11B1 antibody
    • HSD11L antibody
    • Hydroxysteroid (11 beta) dehydrogenase antibody
    • Hydroxysteroid (11 beta) dehydrogenase 1 antibody
    • MGC13539 antibody
    • SDR26C1 antibody
    • short chain dehydrogenase/reductase family 26C, member 1 antibody
    see all

Anti-HSD11B1 antibody images

  • Anti-HSD11B1 antibody (ab83522) at 1 µg/ml (in 5% skim milk/ PBS) + Human fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 32 kDa
    Observed band size : 29 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-HSD11B1 antibody (ab83522) at 1/2000 dilution (in PBS + 0.1% Tween + 2.5% milk for 90 minutes at 22°C)

    Lane 1 : Tissue lysate of Human liver microsomes
    Lane 2 : Tissue lysate of Mouse liver microsomes
    Lane 3 : Tissue lysate of Pig liver microsomes

    Lysates/proteins at 25 µg per lane.

    Secondary
    An HRP-conjugated Donkey anti-rabbit polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 32 kDa
    Observed band size : ~38 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab83522 at 5 µg/ml staining HSD11B1 in Human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ICC/IF image of ab83522 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83522, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-HSD11B1 antibody (ab83522)

This product has been referenced in:
  • Hintzpeter J  et al. Green tea and one of its constituents, Epigallocatechine-3-gallate, are potent inhibitors of human 11ß-hydroxysteroid dehydrogenase type 1. PLoS One 9:e84468 (2014). WB . Read more (PubMed: 24404164) »
  • Tiganescu A  et al. Increased glucocorticoid activation during mouse skin wound healing. J Endocrinol 221:51-61 (2014). Read more (PubMed: 24464022) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Pig Tissue lysate - other (liver microsomes)
Loading amount 25 µg
Specification liver microsomes
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Sep 10 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (human endometrial stromal cells)
Loading amount 30 µg
Specification human endometrial stromal cells
Treatment cAMP+MPA for a total 8 days
Gel Running Conditions Reduced Denaturing (14%- 85v for 30 mins then 125 v then wet transfer)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. madhuri salker

Verified customer

Submitted Aug 03 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"