1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
We do not guarantee IHC-P for mouse and rat.
Is unsuitable for Flow Cyt,ICC/IF or IP.
Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7-ketocholesterol to 7-beta-hydroxycholesterol.
Widely expressed. Highest expression in liver.
Involvement in disease
Defects in HSD11B1 are a cause of cortisone reductase deficiency (CRD) [MIM:604931]. In CRD, activation of cortisone to cortisol does not occur, resulting in adrenocorticotropin-mediated androgen excess and a phenotype resembling polycystic ovary syndrome (PCOS).
Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Rocamora-Reverte L et al. T-cell autonomous death induced by regeneration of inert glucocorticoid metabolites. Cell Death Dis8:e2948 (2017).
Read more (PubMed: 28726773) »
Brust KB et al. Expression of gluconeogenic enzymes and 11ß-hydroxysteroid dehydrogenase type 1 in liver of diabetic mice after acute exercise. Diabetes Metab Syndr Obes7:495-504 (2014).
Read more (PubMed: 25364268) »