The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200. Predicted molecular weight: 44 kDa.
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Catalyzes the conversion of cortisol to the inactive metabolite cortisone. Modulates intracellular glucocorticoid levels, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids.
Found in placenta, kidney, pancreas, prostate, ovary, small intestine and colon.
Involvement in disease
Defects in HSD11B2 are the cause of apparent mineralocorticoid excess (AME) [MIM:218030]. AME is a potentially fatal disease characterized by severe juvenile low-renin hypertension, sodium retention, hypokalemia and low levels of aldosterone. It often leads to nephrocalcinosis.
Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Immunohistochemistry Image of Human Kidney (formalin-fixed, paraffin-embedded) stained with HSD11B2 antibody (ab115696) at 10 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.