Overview

  • Product nameAnti-HSD11B2 antibody
    See all HSD11B2 primary antibodies
  • Description
    Rabbit polyclonal to HSD11B2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Pig, Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human HSD11B2.

  • Positive control
    • This antibody gave a positive signal in both Human Placenta and Kidney tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab117886 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 44 kDa).

Target

  • FunctionCatalyzes the conversion of cortisol to the inactive metabolite cortisone. Modulates intracellular glucocorticoid levels, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids.
  • Tissue specificityFound in placenta, kidney, pancreas, prostate, ovary, small intestine and colon.
  • Involvement in diseaseDefects in HSD11B2 are the cause of apparent mineralocorticoid excess (AME) [MIM:218030]. AME is a potentially fatal disease characterized by severe juvenile low-renin hypertension, sodium retention, hypokalemia and low levels of aldosterone. It often leads to nephrocalcinosis.
  • Sequence similaritiesBelongs to the short-chain dehydrogenases/reductases (SDR) family.
  • Cellular localizationMicrosome. Endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • 11 beta HSD2 antibody
    • 11 beta hydroxysteroid dehydrogenase type 2 antibody
    • 11 DH2 antibody
    • 11-beta-HSD2 antibody
    • 11-beta-hydroxysteroid dehydrogenase type 2 antibody
    • 11-DH2 antibody
    • AME antibody
    • AME1 antibody
    • Corticosteroid 11 beta dehydrogenase isozyme 2 antibody
    • Corticosteroid 11-beta-dehydrogenase isozyme 2 antibody
    • DHI2_HUMAN antibody
    • HSD11B2 antibody
    • HSD11K antibody
    • HSD2 antibody
    • Hydroxysteroid 11 beta dehydrogenase 2 antibody
    • Hydroxysteroid 11 beta dehydrogenase isoenzyme 2 antibody
    • NAD dependent 11 beta hydroxysteroid dehydrogenase antibody
    • NAD-dependent 11-beta-hydroxysteroid dehydrogenase antibody
    • SDR9C3 antibody
    • Short chain dehydrogenase/reductase family 9C, member 3 antibody
    see all

Anti-HSD11B2 antibody images

  • All lanes : Anti-HSD11B2 antibody (ab117886) at 1 µg/ml

    Lane 1 : Human placenta tissue lysate - total protein (ab29745)
    Lane 2 : Human kidney tissue lysate - total protein (ab30203)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 44 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 13 kDa,150 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

References for Anti-HSD11B2 antibody (ab117886)

ab117886 has not yet been referenced specifically in any publications.

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