Overview

  • Product name
    Anti-HSD17B1 antibody [EP1682Y]
    See all HSD17B1 primary antibodies
  • Description
    Rabbit monoclonal [EP1682Y] to HSD17B1
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cyt, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human HSD17B1 (C terminal). The exact sequence is proprietary.

  • Positive control
    • WB: Human placenta lysate. IHC-P: Human placenta tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab51045 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
ICC/IF 1/100 - 1/250.
Flow Cyt Use 1µg for 106 cells.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/100.
IHC-P 1/100 - 1/250.

Target

  • Function
    Favors the reduction of estrogens and androgens. Also has 20-alpha-HSD activity. Uses preferentially NADH.
  • Pathway
    Steroid biosynthesis; estrogen biosynthesis.
  • Sequence similarities
    Belongs to the short-chain dehydrogenases/reductases (SDR) family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 17 beta HSD 1 antibody
    • 17 beta hydroxysteroid dehydrogenase type 1 antibody
    • 17-beta-HSD 1 antibody
    • 17-beta-hydroxysteroid dehydrogenase type 1 antibody
    • 20 alpha-hydroxysteroid dehydrogenase antibody
    • 20-alpha-HSD antibody
    • DHB1_HUMAN antibody
    • E17KSR antibody
    • E2DH antibody
    • EDH17B1 antibody
    • EDH17B2 antibody
    • EDHB17 antibody
    • Estradiol 17 beta dehydrogenase 1 antibody
    • Estradiol 17-beta-dehydrogenase 1 antibody
    • HSD17 antibody
    • HSD17B1 antibody
    • Hydroxysteroid (17 beta) dehydrogenase 1 antibody
    • MGC13814 antibody
    • Placental 17 beta hydroxysteroid dehydrogenase antibody
    • Placental 17-beta-hydroxysteroid dehydrogenase antibody
    • SDR28C1 antibody
    • Short chain dehydrogenase/reductase family 28CE,member 1 antibody
    see all

Images

  • Anti-HSD17B1 antibody [EP1682Y] (ab51045) at 1/100000 dilution + Human placenta lysate at 10 µg

    Secondary
    Goat anti-Rabbit HRP labeled. at 1/2000 dilution

    Predicted band size : 35 kDa
    Observed band size : 35 kDa
  • Ab51045 (1/100 dilution) staining human HSD17B1 in paraffin embedded human placenta tissue by Immunohistochemistry.
  • Overlay histogram showing JEG3 cells stained with ab51045 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51045, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.

References

This product has been referenced in:
  • Sinreih M  et al. The Significance of the Sulfatase Pathway for Local Estrogen Formation in Endometrial Cancer. Front Pharmacol 8:368 (2017). Read more (PubMed: 28690541) »
  • Rižner TL Estrogen biosynthesis, phase I and phase II metabolism, and action in endometrial cancer. Mol Cell Endocrinol 381:124-39 (2013). Human . Read more (PubMed: 23911898) »

See all 7 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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