Overview

  • Product nameAnti-HSD17B12 antibody
    See all HSD17B12 primary antibodies
  • Description
    Mouse monoclonal to HSD17B12
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human, Recombinant Fragment
    Predicted to work with: Rat, Cow, Pig, Cynomolgus Monkey
  • Immunogen

    Recombinant fragment: SATKTFVDFF SQCLHEEYRS KGVFVQSVLP YFVATKLAKI RKPTLDKPSP ETFVKSAIKT VGLQSRTNG, corresponding to amino acids 203-272 of Human HSD17B12, with a 26 kDa tag. NP_057226

  • Positive control
    • Jurkat cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab88133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 34 kDa.
ELISA Use at an assay dependent dilution.

Target

  • FunctionCatalyzes the transformation of estrone (E1) into estradiol (E2), suggesting a central role in estrogen formation. Its strong expression in ovary and mammary gland suggest that it may constitute the major enzyme responsible for the conversion of E1 to E2 in women. Also has 3-ketoacyl-CoA reductase activity, reducing both long chain 3-ketoacyl-CoAs and long chain fatty acyl-CoAs, suggesting a role in long fatty acid elongation.
  • Tissue specificityExpressed in most tissues tested. Highly expressed in the ovary and mammary. Expressed in platelets.
  • PathwaySteroid biosynthesis; estrogen biosynthesis.
    Lipid metabolism; fatty acid biosynthesis.
  • Sequence similaritiesBelongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.
  • DomainThe di-lysine motif confers endoplasmic reticulum localization for type I membrane proteins.
  • Cellular localizationEndoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 12,3-ketoacyl-CoA reductase,short chain antibody
    • 17 beta HSD 12 antibody
    • 17 beta hydroxysteroid dehydrogenase 12 antibody
    • 17-@beta-HSD XII antibody
    • 17-@beta-hydroxysteroid dehydrogenase XII antibody
    • 17-beta-HSD 12 antibody
    • 17-beta-hydroxysteroid dehydrogenase 12 antibody
    • 17beta HSD type 12 antibody
    • 3 ketoacyl CoA reductase antibody
    • 3-ketoacyl-CoA reductase antibody
    • DHB12_HUMAN antibody
    • Estradiol 17 beta dehydrogenase 12 antibody
    • Estradiol 17-beta-dehydrogenase 12 antibody
    • HSD17B12 antibody
    • Hydroxysteroid (17-beta) dehydrogenase 12 antibody
    • KAR antibody
    • KIK-I antibody
    • Kik1 antibody
    • SDR12C1 antibody
    • Short chain dehydrogenase/reductase family 12C member 1 antibody
    • Steroid dehydrogenase homolog antibody
    see all

Anti-HSD17B12 antibody images

  • Anti-HSD17B12 antibody (ab88133) at 1/500 dilution + Jurkat cell lysate at 25 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 34 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)

References for Anti-HSD17B12 antibody (ab88133)

ab88133 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"