Overview

  • Product name
  • Description
    Rabbit polyclonal to HSD17B13
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    antigen, corresponding to amino acids 119-247 of Human HSD17B13 according to Q7Z5P4.

  • Positive control
    • Human testis tissue, Human Liver lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab122036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/250 - 1/500.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in golgi apparatus and vesicles. Recommended concentration of ab122036 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Lane 1: Mouse liver tissue lysate
    Lane 2: Rat liver tissue lysate
  • ab122036, at 1/400, staining HSD17B13 in paraffin embedded Human testis tissue by Immunohistochemistry.
  • All lanes : Anti-HSD17B13 antibody (ab122036) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique

References

This product has been referenced in:
  • Su W  et al. Comparative proteomic study reveals 17ß-HSD13 as a pathogenic protein in nonalcoholic fatty liver disease. Proc Natl Acad Sci U S A 111:11437-42 (2014). Human . Read more (PubMed: 25028495) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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