Overview

  • Product nameAnti-HSD17B2 antibody
    See all HSD17B2 primary antibodies
  • Description
    Rabbit polyclonal to HSD17B2
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 270-300 of Human HSD17B2

  • Positive control
    • MDA-MB435 cell line lysates, Human prostate carcinoma

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody is purified through a protein A column, followed by peptide affinity purification.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab103665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 43 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionCapable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. Also has 20-alpha-HSD activity. Uses NADH while EDH17B3 uses NADPH.
  • Sequence similaritiesBelongs to the short-chain dehydrogenases/reductases (SDR) family.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 17 beta HSD 2 antibody
    • 17-beta-HSD 2 antibody
    • 17-beta-hydroxysteroid dehydrogenase type 2 antibody
    • 20 alpha HSD antibody
    • 20 alpha hydroxysteroid dehydrogenase antibody
    • 20 alpha-hydroxysteroid dehydrogenase antibody
    • 20-alpha-HSD antibody
    • DHB2_HUMAN antibody
    • E2DH antibody
    • EDH17B2 antibody
    • Estradiol 17-beta-dehydrogenase 2 antibody
    • HSD17 antibody
    • Hsd17b2 antibody
    • Hydroxysteroid (17 beta) dehydrogenase 2 antibody
    • Microsomal 17 beta hydroxysteroid dehydrogenase antibody
    • Microsomal 17-beta-hydroxysteroid dehydrogenase antibody
    • SDR9C2 antibody
    • Short chain dehydrogenase/reductase family 9C, member 2 antibody
    • Testosterone 17 beta dehydrogenase antibody
    • Testosterone 17-beta-dehydrogenase antibody
    see all

Anti-HSD17B2 antibody images

  • Anti-HSD17B2 antibody (ab103665) at 1/100 dilution + MDA-MB435 cell line lysates at 35 µg

    Predicted band size : 43 kDa
  • IHC analysis of HSD17B2 expression in formalin fixed and paraffin embedded Human prostate carcinoma using 1/50 ab103665 followed by peroxidase conjugation of the secondary antibody and DAB staining.

References for Anti-HSD17B2 antibody (ab103665)

ab103665 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Cow Tissue lysate - other (Corpus luteum)
Specification Corpus luteum
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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Submitted Jul 29 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"