Overview

  • Product nameAnti-HSD17B6 antibody
    See all HSD17B6 primary antibodies
  • Description
    Rabbit polyclonal to HSD17B6
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a sequence within N terminal amino acids 1-50 of Human HSD17B6 (MWLYLAAFVG LYYLLHWYRE RQVVSHLQDK YVFITGCDSG FGNLLARQLD)

  • Positive control
    • A172 cells. Liver tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab62221 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).Can be blocked with HSD17B6 peptide (ab156928). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • FunctionNAD-dependent oxidoreductase with broad substrate specificity that shows both oxidative and reductive activity (in vitro). Has 17-beta-hydroxysteroid dehydrogenase activity towards various steroids (in vitro). Converts 5-alpha-androstan-3-alpha,17-beta-diol to androsterone and estradiol to estrone (in vitro). Has 3-alpha-hydroxysteroid dehydrogenase activity towards androsterone (in vitro). Has retinol dehydrogenase activity towards all-trans-retinol (in vitro). Can convert androsterone to epi-androsterone. Androsterone is first oxidized to 5-alpha-androstane-3,17-dione and then reduced to epi-andosterone. Can act on both C-19 and C-21 3-alpha-hydroxysteroids.
  • Tissue specificityDetected in liver and prostate (at protein level). Detected in adult liver, lung, brain, placenta, prostate, adrenal gland, testis, mammary gland, spleen, spinal cord and uterus. Detected in caudate nucleus, and at lower levels in amygdala, corpus callosum, hippocampus, substantia nigra and thalamus. Detected in fetal lung, liver and brain.
  • Sequence similaritiesBelongs to the short-chain dehydrogenases/reductases (SDR) family.
  • Cellular localizationMicrosome membrane. Early endosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 17-beta-HSD 6 antibody
    • 17-beta-HSD6 antibody
    • 17-beta-hydroxysteroid dehydrogenase type 6 antibody
    • 3 hydroxysteroid epimerase antibody
    • 3(alpha >beta) hydroxysteroid epimerase antibody
    • 3(alpha >beta) hydroxysteroid epimerasel antibody
    • 3-alpha->beta-HSE antibody
    • 3-alpha->beta-hydroxysteroid epimerase antibody
    • H17B6_HUMAN antibody
    • HSD17B6 antibody
    • HSE antibody
    • Hydroxysteroid (17 beta) dehydrogenase 6 antibody
    • Hydroxysteroid (17 beta) dehydrogenase 6 homolog (mouse) antibody
    • Hydroxysteroid 17 beta dehydrogenase 6 antibody
    • NAD+ dependent 3 alpha hydroxysteroid dehydrogenase 3 hydroxysteroid epimerase antibody
    • NAD+ dependent 3 alpha hydroxysteroid dehydrogenase antibody
    • Oxidative 3 alpha hydroxysteroid dehydrogenase antibody
    • Oxidative 3-alpha hydroxysteroid dehydrogenase antibody
    • Oxidoreductase antibody
    • Retinol dehydrogenase antibody
    • RODH antibody
    • SDR9C6 antibody
    • Short chain dehydrogenase/reductase family 9C, member 6 antibody
    see all

Anti-HSD17B6 antibody images

  • Anti-HSD17B6 antibody (ab62221) at 1.25 µg/ml + A172 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 35 kDa
    Observed band size : 35 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling HSD17B6 with ab62221 at 4-8µg/ml. Arrows indicate positively labelled hemopoietic cells. Magnification: 400X.

References for Anti-HSD17B6 antibody (ab62221)

This product has been referenced in:
  • Mohler JL  et al. Activation of the androgen receptor by intratumoral bioconversion of androstanediol to dihydrotestosterone in prostate cancer. Cancer Res 71:1486-96 (2011). WB . Read more (PubMed: 21303972) »
  • Muthusamy S  et al. Estrogen receptor ß and 17ß-hydroxysteroid dehydrogenase type 6, a growth regulatory pathway that is lost in prostate cancer. Proc Natl Acad Sci U S A 108:20090-4 (2011). IHC-P ; Human . Read more (PubMed: 22114194) »

See all 2 Publications for this product

Product Wall

The peptide to ab62221 has been added as HSD17B6 peptide (ab156928)- http://www.abcam.com/hsd17b6-peptide-ab156928.html

Thank you for your response and for attaching the Western blot images.

There is a clear difference in the banding pattern between cell prepared with RIPA buffer and those with Laemmli lysis buffer. This highlights the importance of use of pro...

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Thank you for your enquiry regarding ab62221 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

As our Abpromise indicates, in the event that a p...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

We are expecting the return of the 2 vials of ab62221 to our Cambridge, MA office. As requested, ...

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Thank you for your enquiry regarding ab62221. The expected band size of HSD17B6 protein is around 35 kDa, however several factors may influence the actual band size. Could you provide some further details of the protocol used and complete the f...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"