Overview

  • Product nameAnti-HSD17B6 antibody
    See all HSD17B6 primary antibodies
  • Description
    Mouse polyclonal to HSD17B6
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human HSD17B6 protein (NP_003716.2).

  • Positive control
    • Human liver tissue lysate; HSD17B6 transfected 293T cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1 x PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab88893 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa.

Target

  • FunctionNAD-dependent oxidoreductase with broad substrate specificity that shows both oxidative and reductive activity (in vitro). Has 17-beta-hydroxysteroid dehydrogenase activity towards various steroids (in vitro). Converts 5-alpha-androstan-3-alpha,17-beta-diol to androsterone and estradiol to estrone (in vitro). Has 3-alpha-hydroxysteroid dehydrogenase activity towards androsterone (in vitro). Has retinol dehydrogenase activity towards all-trans-retinol (in vitro). Can convert androsterone to epi-androsterone. Androsterone is first oxidized to 5-alpha-androstane-3,17-dione and then reduced to epi-andosterone. Can act on both C-19 and C-21 3-alpha-hydroxysteroids.
  • Tissue specificityDetected in liver and prostate (at protein level). Detected in adult liver, lung, brain, placenta, prostate, adrenal gland, testis, mammary gland, spleen, spinal cord and uterus. Detected in caudate nucleus, and at lower levels in amygdala, corpus callosum, hippocampus, substantia nigra and thalamus. Detected in fetal lung, liver and brain.
  • Sequence similaritiesBelongs to the short-chain dehydrogenases/reductases (SDR) family.
  • Cellular localizationMicrosome membrane. Early endosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 17-beta-HSD 6 antibody
    • 17-beta-HSD6 antibody
    • 17-beta-hydroxysteroid dehydrogenase type 6 antibody
    • 3 hydroxysteroid epimerase antibody
    • 3(alpha >beta) hydroxysteroid epimerase antibody
    • 3(alpha >beta) hydroxysteroid epimerasel antibody
    • 3-alpha->beta-HSE antibody
    • 3-alpha->beta-hydroxysteroid epimerase antibody
    • H17B6_HUMAN antibody
    • HSD17B6 antibody
    • HSE antibody
    • Hydroxysteroid (17 beta) dehydrogenase 6 antibody
    • Hydroxysteroid (17 beta) dehydrogenase 6 homolog (mouse) antibody
    • Hydroxysteroid 17 beta dehydrogenase 6 antibody
    • NAD+ dependent 3 alpha hydroxysteroid dehydrogenase 3 hydroxysteroid epimerase antibody
    • NAD+ dependent 3 alpha hydroxysteroid dehydrogenase antibody
    • Oxidative 3 alpha hydroxysteroid dehydrogenase antibody
    • Oxidative 3-alpha hydroxysteroid dehydrogenase antibody
    • Oxidoreductase antibody
    • Retinol dehydrogenase antibody
    • RODH antibody
    • SDR9C6 antibody
    • Short chain dehydrogenase/reductase family 9C, member 6 antibody
    see all

Anti-HSD17B6 antibody images

  • Anti-HSD17B6 antibody (ab88893) at 1 µg/ml + human liver tissue lysate at 50 µg
    Developed using the ECL technique

    Predicted band size : 36 kDa
    Observed band size : ~30 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-HSD17B6 antibody (ab88893) at 1 µg/ml

    Lane 1 : HSD17B6 transfected 293T cell lysate
    Lane 2 : non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Developed using the ECL technique

    Predicted band size : 36 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 25 kDa,~30 kDa. We are unsure as to the identity of these extra bands.

References for Anti-HSD17B6 antibody (ab88893)

ab88893 has not yet been referenced specifically in any publications.

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