Recombinant
RabMAb

Anti-HSD17B8 antibody [EPR12084(B)] (ab169752)

Overview

  • Product name
    Anti-HSD17B8 antibody [EPR12084(B)]
    See all HSD17B8 primary antibodies
  • Description
    Rabbit monoclonal [EPR12084(B)] to HSD17B8
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to amino acid residues in Human HSD17B8 (Q92506).

  • Positive control
    • Human fetal liver, HepG2, BxPC-3 and JAR cell lysates. JAR cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab169752 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/10000 - 1/50000. Predicted molecular weight: 26 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function
      NAD-dependent 17-beta-hydroxysteroid dehydrogenase with highest activity towards estradiol. Has very low activity towards testosterone. The heteroteramer with CBR4 has NADH-dependent 3-ketoacyl-acyl carrier protein reductase activity. May play a role in biosynthesis of fatty acids in mitochondria.
    • Tissue specificity
      Highly expressed in placenta, liver and pancreas, lower in the skeletal muscle and kidney. Widely expressed.
    • Pathway
      Steroid biosynthesis; estrogen biosynthesis.
      Lipid metabolism; fatty acid biosynthesis.
    • Sequence similarities
      Belongs to the short-chain dehydrogenases/reductases (SDR) family.
    • Cellular localization
      Mitochondrion matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • 17 beta HSD 8 antibody
      • 17 beta hydroxysteroid dehydrogenase 8 antibody
      • 17-beta-HSD 8 antibody
      • 17-beta-hydroxysteroid dehydrogenase 8 antibody
      • 17-beta-hydroxysteroid dehydrogenase VIII antibody
      • 3-oxoacyl-[acyl-carrier-protein] reductase antibody
      • 3-oxoacyl-acyl-carrier protein reductase, E. coli, homolog of antibody
      • Beta ketoacyl [acyl carrier protein] reductase like antibody
      • D6S2245E antibody
      • DHB8_HUMAN antibody
      • dJ1033B10.9 antibody
      • Estradiol 17 beta dehydrogenase 8 antibody
      • Estradiol 17-beta-dehydrogenase 8 antibody
      • Estrogen 17 oxidoreductase antibody
      • FABG antibody
      • FabG, E. coli, homolog of antibody
      • FabG-like antibody
      • FABGL antibody
      • H2 KE6 antibody
      • H2-KE6 antibody
      • HKE6 antibody
      • HSD17B8 antibody
      • Hydroxysteroid (17 beta) dehydrogenase 8 antibody
      • Ke-6 antibody
      • KE6 antibody
      • KE6, mouse homolog of antibody
      • Protein Ke6 antibody
      • Really interesting new gene 2 protein antibody
      • RING2 antibody
      • SDR30C1 antibody
      • Short chain dehydrogenase/reductase family 30C member 1 antibody
      • Testosterone 17 beta dehydrogenase 8 antibody
      • Testosterone 17-beta-dehydrogenase 8 antibody
      see all

    Images

    • Immunofluorescent analysis of JAR cells labelling HSD17B8 with ab169752 at 1/100 dilution.
    • All lanes : Anti-HSD17B8 antibody [EPR12084(B)] (ab169752) at 1/10000 dilution

      Lane 1 : Fetal liver lysate
      Lane 2 : HepG2 lysate
      Lane 3 : Bx-PC-3 lysate
      Lane 4 : JAR lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 26 kDa

    • Western blot analysis on immunoprecipitation pellet from (1) fetal liver lysate labeling HSD17B8 with ab169752 at 1/10 dilution or (2) 1X PBS (negative control. HRP-conjugated anti-rabbit IgG preferentially detected the non-reduced form of Rabbit IgG.

    References

    ab169752 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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