Overview

  • Product nameAnti-HSD3a antibody
    See all HSD3a primary antibodies
  • Description
    Goat polyclonal to HSD3a
  • SpecificityThis antibody may cross-react with AKR1C1.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cynomolgus Monkey, Macaque Monkey
  • Immunogen

    Synthetic peptide:

    C-DPKYQRVELNDGH

    , corresponding to N terminal amino acids 2-14 of Human HSD3a

  • Positive control
    • Human Liver lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab50415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 37 kDa).
ELISA Use at an assay dependent dilution.
IHC-P Use a concentration of 2 - 4 µg/ml.

Target

  • FunctionCatalyzes the transformation of the potent androgen dihydrotestosterone (DHT) into the less active form, 5-alpha-androstan-3-alpha,17-beta-diol (3-alpha-diol). Also has some 20-alpha-hydroxysteroid dehydrogenase activity. The biotransformation of the pesticide chlordecone (kepone) to its corresponding alcohol leads to increased biliary excretion of the pesticide and concomitant reduction of its neurotoxicity since bile is the major excretory route.
  • Tissue specificityLiver specific.
  • Sequence similaritiesBelongs to the aldo/keto reductase family.
  • Post-translational
    modifications
    The N-terminus is blocked.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3 alpha HSD1 antibody
    • 3-alpha-HSD1 antibody
    • 3-alpha-hydroxysteroid dehydrogenase type I antibody
    • AK1C4_HUMAN antibody
    • AKR1C4 antibody
    • Aldo keto reductase family 1 member C4 antibody
    • Aldo-keto reductase family 1 member C4 antibody
    • CDR antibody
    • Chlordecone reductase antibody
    • DD-4 antibody
    • DD4 antibody
    • Dihydrodiol dehydrogenase 4 antibody
    • HAKRA antibody
    see all

Anti-HSD3a antibody images

  • Anti-HSD3a antibody (ab50415) at 0.1 µg/ml + Human Liver lysate (35µg protein in RIPA buffer).

    Predicted band size : 37 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • ab50415 at 4µg/ml staining HSD3a in Human liver tissue section by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The tissue underwent antigen retrieval by steam in citrate buffer pH 6.0.

References for Anti-HSD3a antibody (ab50415)

ab50415 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"