• Product nameAnti-HSD3B1 antibody
    See all HSD3B1 primary antibodies
  • Description
    Mouse monoclonal to HSD3B1
  • Tested applicationsSuitable for: Flow Cyt, WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-374 of Human HSD3B1



Our Abpromise guarantee covers the use of ab55268 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
IHC-P Use a concentration of 5 µg/ml.
IHC-Fr Use at an assay dependent concentration. PubMed: 21094146


  • Function3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. Efficiently catalyzes the transformation of pregnenolone to progesterone, 17-alpha-hydroxypregnenolone to 17-alpha-hydroxyprogesterone, DHEA to 4-androstenedione, dihydrotestosterone to 5-alpha-androstane-3 beta,17 beta-diol, dehydroepiandrosterone to androstenedione and 5-alpha-androstan-3 beta,17 beta-diol to 5-alpha-dihydrotestosterone.
  • Tissue specificityPlacenta and skin. Predominantly expressed in mammary gland tissue.
  • PathwayLipid metabolism; steroid biosynthesis.
  • Sequence similaritiesBelongs to the 3-beta-HSD family.
  • Cellular localizationEndoplasmic reticulum membrane. Mitochondrion membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3 beta and steroid delta isomerase 1 antibody
    • 3 beta and steroid hydroxy delta 5 steroid dehydrogenase antibody
    • 3 beta HSD I antibody
    • 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I antibody
    • 3-beta-HSD I antibody
    • 3-beta-hydroxy-5-ene steroid dehydrogenase antibody
    • 3BETAHSD antibody
    • 3BH antibody
    • 3BHS1_HUMAN antibody
    • 3BHSD antibody
    • Delta-5-3-ketosteroid isomerase antibody
    • HGNC:5217 antibody
    • HSD3B antibody
    • HSD3B1 antibody
    • HSDB3 antibody
    • HSDB3A antibody
    • Hydroxy delta 5 steroid dehydrogenase 3 beta and steroid delta isomerase 1 antibody
    • Hydroxy delta 5 steroid dehydrogenase antibody
    • Progesterone reductase antibody
    • SDR11E1 antibody
    • Steroid Delta-isomerase antibody
    • trophoblast antigen antibody
    • Trophoblast antigen FDO161G antibody
    see all

Anti-HSD3B1 antibody images

  • HSD3B1 antibody (ab55268) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human placenta.
  • Anti-HSD3B1 antibody (ab55268) at 1 µg/ml + Human placenta tissue lysate - total protein (ab29745) at 10 µg

    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 42 kDa
    Additional bands at : 73 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
  • Overlay histogram showing JEG3 cells stained with ab55268 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55268, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in JEG3 cells fixed with 80% methanol/permeabilized in 0.1% PBS-Tween used under the same conditions.

References for Anti-HSD3B1 antibody (ab55268)

This product has been referenced in:
  • Maingat FG  et al. Neurosteroid-mediated regulation of brain innate immunity in HIV/AIDS: DHEA-S suppresses neurovirulence. FASEB J : (2012). Read more (PubMed: 23150523) »
  • Hannen RF  et al. Steroid synthesis by primary human keratinocytes; implications for skin disease. Biochem Biophys Res Commun 404:62-7 (2011). WB, IHC-Fr ; Human . Read more (PubMed: 21094146) »

See all 2 Publications for this product

Product Wall

Anti-HSD3B1 antibody (ab55268) is raised against the full length protein, corresponding to amino acids 1-374 of Human HSD3B1.

Sequence alignment of Human HSD3B1 against HSD3B2 is 94%.

This suggests that ab55268 is not specific to HSD...

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Thank you for your reply.

We recommend using 5% milk or BSA for the blocking step, however, when incubating with the primary antibody we recommend reducing that concentration to 1% or less. Blocking agent is not strictly necessary in the anti...

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Thank you for contacting us and for forwarding the customer's protocol information. I am sorry that this antibody is not producing satisfactory results in WB. Could you please provide some additional information about the experiment?

1) Were ...

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Thank you for filling in the questionnaire and for reporting this problem to us. We will use the details you sent about your protocol in order to investigate the source of the problem. I have looked through the protocol, which looks fine to me....

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Thank you for contacting us. The HSD3B1 protein has 71% similarity between human and mouse. Theoretically the antibody should not be detecting the mouse protein however because we have not tested this so will not be able to confirm it. http://w...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rhesus monkey Tissue sections (Testis)
Specification Testis
Fixative Formaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Aug 09 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Placenta)
Specification Placenta
Fixative Formaldehyde
Antigen retrieval step None
Permeabilization No

Abcam user community

Verified customer

Submitted Jul 20 2009