Overview

  • Product nameAnti-HSD3B2 antibody
    See all HSD3B2 primary antibodies
  • Description
    Rabbit polyclonal to HSD3B2
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Sheep, Goat, Horse, Cow, Cat, Dog, Chimpanzee, Macaque Monkey
  • Immunogen

    Synthetic peptide: MGWSCLVTGA GGLLGQRIVR LLVEEKELKE IRALDKAFRP ELREEFSKLQ, corresponding to N terminal amino acids 1-50 of Human HSD3B2

  • Positive control
    • 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab80500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.
  • Tissue specificityExpressed in adrenal gland, testis and ovary.
  • PathwayLipid metabolism; steroid biosynthesis.
  • Involvement in diseaseDefects in HSD3B2 are the cause of adrenal hyperplasia type 2 (AH2) [MIM:201810]. AH2 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: 'salt wasting' (SW, the most severe type), 'simple virilizing' (SV, less severely affected patients), with normal aldosterone biosynthesis, 'non-classic form' or late onset (NC or LOAH), and 'cryptic' (asymptomatic). In AH2, virilization is much less marked or does not occur. AH2 is frequently lethal in early life.
    Note=Mild HSD3B2 deficiency in hyperandrogenic females is associated with characteristic traits of polycystic ovary syndrome, such as insulin resistance and luteinizing hormon hypersecretion.
  • Sequence similaritiesBelongs to the 3-beta-HSD family.
  • Cellular localizationEndoplasmic reticulum membrane. Mitochondrion membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3 beta HSD adrenal and gonadal type antibody
    • 3 beta HSD II antibody
    • 3 beta HSD type II antibody
    • 3 beta hydroxy 5 ene steroid dehydrogenase antibody
    • 3 beta hydroxy Delta(5) steroid dehydrogenase antibody
    • 3 beta hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2 antibody
    • 3 beta-hydroxysteroid dehydrogenase type II, delta 5-delta 4-isomerase type II, 3 beta-HSD type II antibody
    • 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II antibody
    • 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2 antibody
    • 3-beta-HSD II antibody
    • 3-beta-hydroxy-5-ene steroid dehydrogenase antibody
    • 3-beta-hydroxy-Delta(5)-steroid dehydrogenase antibody
    • 3BHS2_HUMAN antibody
    • ADRENAL HYPERPLASIA II antibody
    • beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2 antibody
    • delta 5 delta 4 isomerase type II antibody
    • Delta-5-3-ketosteroid isomerase antibody
    • HSD3B antibody
    • HSD3B2 antibody
    • HSDB antibody
    • HSDB3B antibody
    • hydroxy delta 5 steroid dehydrogenase, 3 beta and steroid delta isomerase 2 antibody
    • Hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2 antibody
    • Progesterone reductase antibody
    • SDR11E2 antibody
    • short chain dehydrogenase/reductase family 11E, member 2 antibody
    • Steroid Delta-isomerase antibody
    see all

Anti-HSD3B2 antibody images

  • Anti-HSD3B2 antibody (ab80500) at 1 µg/ml + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 42 kDa
  • ICC/IF image of ab80500 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80500, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-HSD3B2 antibody (ab80500)

This product has been referenced in:
  • Verhagen I  et al. Time course and role of luteinizing hormone and follicle-stimulating hormone in the expansion of the Leydig cell population at the time of puberty in the rhesus monkey (Macaca mulatta). Andrology 2:924-30 (2014). Read more (PubMed: 25269763) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"