Anti-HSDL2 antibody [2A11BG6AH6] (ab110298)

Overview

  • Product name
    Anti-HSDL2 antibody [2A11BG6AH6]
    See all HSDL2 primary antibodies
  • Description
    Mouse monoclonal [2A11BG6AH6] to HSDL2
  • Tested applications
    Suitable for: ICC, IP, In-Cell ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human liver mitochondria.

  • Positive control
    • Hela cell and human liver mitochondria.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituent: HBS
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Purification notes
    Purity near homogeneity as judge by SDS-PAGE. The antibody was produced in-vitro using hybridomas grown in serum-free medium and then purified by biochemical fractionation
  • Clonality
    Monoclonal
  • Clone number
    2A11BG6AH6
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab110298 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 10 µg/ml.
IP Use at an assay dependent dilution.
In-Cell ELISA Use a concentration of 0.4 µg/ml. (0.4 µg/well)

Target

  • Function
    Has apparently no steroid dehydrogenase activity.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the short-chain dehydrogenases/reductases (SDR) family.
    Contains 1 SCP2 domain.
  • Cellular localization
    Peroxisome.
  • Information by UniProt
  • Database links
  • Alternative names
    • C9orf99 antibody
    • FLJ25855 antibody
    • hsdl2 antibody
    • HSDL2_HUMAN antibody
    • Hydroxysteroid dehydrogenase like protein 2 antibody
    • Hydroxysteroid dehydrogenase-like protein 2 antibody
    • MGC10940 antibody
    • MS728 antibody
    • SDR13C1 antibody
    • Short chain dehydrogenase/reductase family 13C, member 1 antibody
    see all

Images

  • As an IP antibody, HSDL2 antibody [2A11BG6AH6 ] (ab110298) works with human sample only.
  • Immunocytochemistry image of HSDL2 stained human Hela cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with HSDL2 antibody [2A11BG6AH6 ](ab110298) at 10 µg/ml for 2 hours at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps.

References

ab110298 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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