Use at an assay dependent concentration. PubMed: 22067449
FunctionHistone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overalpping localization with SETD1B suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression.
Cellular localizationNucleus speckle. Chromosome. Localizes to a largely non-overlapping set of euchromatic nuclear speckles with SETD1B, suggesting that SETD1A and SETD1B each bind to a unique set of target genes.
Lane 1 : 293T whole cell lysate at 100 µg Lane 2 : 293T whole cell lysate at 200 µg Lane 3 : 293T nuclear lysate at 100 µg Lane 4 : 293T nuclear lysate at 200 µg Lane 5 : 293T whole cell lysate at 100 µg Lane 6 : 293T whole cell lysate at 200 µg Lane 7 : 293T nuclear lysate at 100 µg Lane 8 : 293T nuclear lysate at 200 µg
Detection of Human hSET1 by Immunoprecipitation. Control IgG used in lane 1, ab70379 used at 5 µg/5mg nuclear extract from 293T cells in lane 2. Immunoprecipitated hSET1 was detected with post IP WB using ab70379 at 0.2 µg/ml. Detection: Chemiluminescence.
References for Anti-hSET1 antibody (ab70379)
This product has been referenced in:
Le Douce V et al. LSD1 cooperates with CTIP2 to promote HIV-1 transcriptional silencing. Nucleic Acids Res40:1904-15 (2012).
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