Overview

  • Product name
  • Description
    Rabbit polyclonal to hSET1
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residues 1200 and 1250 of Human hSET1, TrEMBL entry O15047

  • Positive control
    • Human breast adenocarcinoma and mouse Squamous cell carcinoma tissues.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab84510 was affinity purified using an epitope specific to hSET1 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
IHC-P 1/100 - 1/500. Epitope exposure with citrate buffer will enhance staining.

Target

  • Function
    Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overalpping localization with SETD1B suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression.
  • Sequence similarities
    Contains 1 post-SET domain.
    Contains 1 RRM (RNA recognition motif) domain.
    Contains 1 SET domain.
  • Cellular localization
    Nucleus speckle. Chromosome. Localizes to a largely non-overlapping set of euchromatic nuclear speckles with SETD1B, suggesting that SETD1A and SETD1B each bind to a unique set of target genes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Histone-lysine N-methyltransferase SETD1A antibody
    • hSET1A antibody
    • KMT2F antibody
    • Lysine N methyltransferase 2F antibody
    • Lysine N-methyltransferase 2F antibody
    • SET domain containing 1A antibody
    • SET domain containing protein 1A antibody
    • SET domain-containing protein 1A antibody
    • SET1 antibody
    • Set1 Ash2 histone methyltransferase complex subunit SET1 antibody
    • Set1/Ash2 histone methyltransferase complex subunit SET1 antibody
    • SET1A antibody
    • SET1A_HUMAN antibody
    • SETD1A antibody
    see all

Images

  • ab84510 at 1/250 dilution staining hSET1 in Human breast adenocarcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
  • ab84510 at 1/250 dilution staining hSET1 in Mouse squamous cell carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
  • ab84510 (1/200) staining hSET1 in assynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please see Abreview.

    See Abreview

References

ab84510 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton X100
Username

Dr. Kirk Mcmanus

Verified customer

Submitted Dec 09 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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