Overview

  • Product nameAnti-HSF1 antibody
    See all HSF1 primary antibodies
  • Description
    Rabbit polyclonal to HSF1
  • Specificityab47484 detects endogenous levels of total HSF1 protein.
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    The antiserum was produced against synthesized non-phosphopeptide derived from human HSF1 around the phosphorylation site of serine 303 (P-P-SP-P-P).

  • Positive control
    • Breast carcinoma tissue. MCF cells extract.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab47484 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 57 kDa.
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.

Target

  • FunctionDNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
  • Sequence similaritiesBelongs to the HSF family.
  • Domainthe 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • Post-translational
    modifications
    Phosphorylated on multiple serine residues, a subset of which are involved in stress-related regulation of transcription activation. Constitutive phosphorylation represses transcriptional activity at normal temperatures. Levels increase on specific residues heat-shock and enhance HSF1 transactivation activity. Phosphorylation on Ser-307 derepresses activation on heat-stress and in combination with Ser-303 phosphorylation appears to be involved in recovery after heat-stress. Phosphorylated on Ser-230 by CAMK2, in vitro. Cadmium also enhances phosphorylation at this site. Phosphorylation on Ser-303 is a prerequisite for HSF1 sumoylation. Phosphorylation on Ser-121 inhibits transactivation and promotes HSP90 binding. Phosphorylation on Thr-142 also mediates transcriptional activity induced by heat. Phosphorylation on Ser-326 plays an important role in heat activation of HSF1 transcriptional activity.
    Sumoylated with SUMO1 and SUMO2 on heat-shock. Heat-inducible sumoylation occurs after 15 min of heat-shock, after which levels decrease and at 4 hours, levels return to control levels. Sumoylation has no effect on HSE binding nor on transcriptional activity. Phosphorylation on Ser-303 is a prerequisite for sumoylation.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules.
  • Information by UniProt
  • Database links
  • Alternative names
    • Heat shock factor 1 antibody
    • Heat shock factor protein 1 antibody
    • Heat shock transcription factor 1 antibody
    • HSF 1 antibody
    • hsf1 antibody
    • HSF1_HUMAN antibody
    • HSTF 1 antibody
    • HSTF1 antibody
    see all

Anti-HSF1 antibody images

  • Staining of HSF1 on paraffin-embedded human breast carcinoma tissue using ab47484 diluted 1/50. Right panel: the anibody was incubated with the corresponding blocking peptide.
  • All lanes : Anti-HSF1 antibody (ab47484) at 1/500 dilution

    Lane 1 : Extracts from MCF cells treated with TNF-alpha (20ng/ml, 30min)
    Lane 2 : Extracts from MCF cells treated with TNF-alpha (20ng/ml, 30min) with blocking peptide


    Predicted band size : 57 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)

References for Anti-HSF1 antibody (ab47484)

This product has been referenced in:
  • Bellingham M  et al. Exposure to chemical cocktails before or after conception - the effect of timing on ovarian development. Mol Cell Endocrinol 376:156-72 (2013). IHC . Read more (PubMed: 23791816) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"