Overview

  • Product name
  • Description
    Rabbit polyclonal to HSF1
  • Specificity
    This antibody reacts with HSF1.
  • Tested applications
    Suitable for: WB, Dot blotmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse HSF1 aa 434-448 conjugated to Keyhole Limpet Haemocyanin (KLH).

  • Positive control
    • HeLa cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab49995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Dot blot
  • Application notes
    Dot: Use at a concentration of 0.5 - 1 µg/ml.
    WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 57 kDa).
    Suitable for array experiments.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
    • Sequence similarities
      Belongs to the HSF family.
    • Domain
      the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
    • Post-translational
      modifications
      Phosphorylated on multiple serine residues, a subset of which are involved in stress-related regulation of transcription activation. Constitutive phosphorylation represses transcriptional activity at normal temperatures. Levels increase on specific residues heat-shock and enhance HSF1 transactivation activity. Phosphorylation on Ser-307 derepresses activation on heat-stress and in combination with Ser-303 phosphorylation appears to be involved in recovery after heat-stress. Phosphorylated on Ser-230 by CAMK2, in vitro. Cadmium also enhances phosphorylation at this site. Phosphorylation on Ser-303 is a prerequisite for HSF1 sumoylation. Phosphorylation on Ser-121 inhibits transactivation and promotes HSP90 binding. Phosphorylation on Thr-142 also mediates transcriptional activity induced by heat. Phosphorylation on Ser-326 plays an important role in heat activation of HSF1 transcriptional activity.
      Sumoylated with SUMO1 and SUMO2 on heat-shock. Heat-inducible sumoylation occurs after 15 min of heat-shock, after which levels decrease and at 4 hours, levels return to control levels. Sumoylation has no effect on HSE binding nor on transcriptional activity. Phosphorylation on Ser-303 is a prerequisite for sumoylation.
    • Cellular localization
      Cytoplasm. Nucleus. Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules.
    • Information by UniProt
    • Database links
    • Alternative names
      • Heat shock factor 1 antibody
      • Heat shock factor protein 1 antibody
      • Heat shock transcription factor 1 antibody
      • HSF 1 antibody
      • hsf1 antibody
      • HSF1_HUMAN antibody
      • HSTF 1 antibody
      • HSTF1 antibody
      see all

    Images

    • Lane 1 : Anti-HSF1 antibody (ab49995) at 1 µg/ml
      Lane 2 : Anti-HSF1 antibody (ab49995) at 0.5 µg/ml
      Lane 3 : Anti-HSF1 antibody (ab49995) at 0.25 µg/ml

      Lane 1 : HeLa cell Lysate
      Lane 2 : HeLa cell Lysate
      Lane 3 : HeLa cell Lysate

      Secondary
      Goat Anti-Rabbit IgG-Peroxidase conjugate

      Predicted band size : 57 kDa
      Observed band size : 63 kDa (why is the actual band size different from the predicted?)

    References

    ab49995 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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