Anti-HSF1 (phospho S307) antibody (ab75905)


  • Product name
    Anti-HSF1 (phospho S307) antibody
    See all HSF1 primary antibodies
  • Description
    Rabbit polyclonal to HSF1 (phospho S307)
  • Specificity
    ab75905 detects endogenous levels of HSF1 only when phosphorylated at serine 307.
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic phosphopeptide derived from human HSF1 around the phosphorylation site of serine 307 (P-Q-SP-P-R).

  • Positive control
    • HUVEC cell extracts treated with TNF-a; HeLa cells.



Our Abpromise guarantee covers the use of ab75905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/200.
WB 1/500 - 1/1000. Predicted molecular weight: 57 kDa.


  • Function
    DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
  • Sequence similarities
    Belongs to the HSF family.
  • Domain
    the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • Post-translational
    Phosphorylated on multiple serine residues, a subset of which are involved in stress-related regulation of transcription activation. Constitutive phosphorylation represses transcriptional activity at normal temperatures. Levels increase on specific residues heat-shock and enhance HSF1 transactivation activity. Phosphorylation on Ser-307 derepresses activation on heat-stress and in combination with Ser-303 phosphorylation appears to be involved in recovery after heat-stress. Phosphorylated on Ser-230 by CAMK2, in vitro. Cadmium also enhances phosphorylation at this site. Phosphorylation on Ser-303 is a prerequisite for HSF1 sumoylation. Phosphorylation on Ser-121 inhibits transactivation and promotes HSP90 binding. Phosphorylation on Thr-142 also mediates transcriptional activity induced by heat. Phosphorylation on Ser-326 plays an important role in heat activation of HSF1 transcriptional activity.
    Sumoylated with SUMO1 and SUMO2 on heat-shock. Heat-inducible sumoylation occurs after 15 min of heat-shock, after which levels decrease and at 4 hours, levels return to control levels. Sumoylation has no effect on HSE binding nor on transcriptional activity. Phosphorylation on Ser-303 is a prerequisite for sumoylation.
  • Cellular localization
    Cytoplasm. Nucleus. Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules.
  • Information by UniProt
  • Database links
  • Alternative names
    • Heat shock factor 1 antibody
    • Heat shock factor protein 1 antibody
    • Heat shock transcription factor 1 antibody
    • HSF 1 antibody
    • hsf1 antibody
    • HSF1_HUMAN antibody
    • HSTF 1 antibody
    • HSTF1 antibody
    see all

Anti-HSF1 (phospho S307) antibody images

  • ab75905 at 1/100 dilution staining HSF1 in methanol-fixed HeLa cells by Immunofluorescence.
  • All lanes : Anti-HSF1 (phospho S307) antibody (ab75905) at 1/500 dilution

    Lane 1 : HUVEC cell extracts treated with TNF-a with immunising peptide
    Lane 2 : HUVEC cell extracts treated with TNF-a

    Predicted band size : 57 kDa
  • ab75905 at 1/200 dilution staining HSF1 (phospho S307) in human Hela cells by ICC/IF.

References for Anti-HSF1 (phospho S307) antibody (ab75905)

ab75905 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab75905.
Please use the links above to contact us or submit feedback about this product.


Sign up